نتایج جستجو برای: exosome activity

تعداد نتایج: 1137073  

Journal: :Npg Asia Materials 2021

Abstract Exosomes have attracted increasing attention in tissue regeneration and repair due to their roles intercellular communication. Developing a customized delivery system is key exosome-based regenerative therapeutics. Bioceramics play an important role the immunomodulation of macrophages. Here, three-dimensional (3D) printing was applied construct porous scaffolds with β-tricalcium phosph...

Journal: :The Journal of biological chemistry 2016
Rajendra K Gangalum Ankur M Bhat Sirus A Kohan Suraj P Bhat

Exosomes carry cell type-specific molecular cargo to extracellular destinations and therefore act as lateral vectors of intercellular communication and transfer of genetic information from one cell to the other. We have shown previously that the small heat shock protein αB-crystallin (αB) is exported out of the adult human retinal pigment epithelial cells (ARPE19) packaged in exosomes. Here, we...

2010
Chieh Hsu Yuichi Morohashi Shin-ichiro Yoshimura Natalia Manrique-Hoyos SangYong Jung Marcel A. Lauterbach Mostafa Bakhti Mads Grønborg Wiebke Möbius JeongSeop Rhee Francis A. Barr Mikael Simons

Oligodendrocytes secrete vesicles into the extracellular space, where they might play a role in neuron-glia communication. These exosomes are small vesicles with a diameter of 50-100 nm that are formed within multivesicular bodies and are released after fusion with the plasma membrane. The intracellular pathways that generate exosomes are poorly defined. Because Rab family guanosine triphosphat...

2016
Sebastian Falk Ksenia Finogenova Mireille Melko Christian Benda Søren Lykke-Andersen Torben Heick Jensen Elena Conti

The eukaryotic RNA exosome participates extensively in RNA processing and degradation. In human cells, three accessory factors (RBM7, ZCCHC8 and hMTR4) interact to form the nuclear exosome targeting (NEXT) complex, which directs a subset of non-coding RNAs for exosomal degradation. Here we elucidate how RBM7 is incorporated in the NEXT complex. We identify a proline-rich segment of ZCCHC8 as th...

2016
Ruenn Chai Lai Soon Sim Tan Ronne Wee Yeh Yeo Andre Boon Hwa Choo Agnes T. Reiner Yan Su Yang Shen Zhiyan Fu Lezhava Alexander Siu Kwan Sze Sai Kiang Lim

Mesenchymal stem cell (MSC), a widely used adult stem cell candidate for regenerative medicine, has been shown to exert some of its therapeutic effects through the secretion of extracellular vesicles (EVs). These homogenously sized EVs of 100-150 ηm exhibited many exosome-like biophysical and biochemical properties and carry both proteins and RNAs. Recently, exosome-associated proteins in this ...

2014
Rafal Tomecki Karolina Drazkowska Iwo Kucinski Krystian Stodus Roman J. Szczesny Jakub Gruchota Ewelina P. Owczarek Katarzyna Kalisiak Andrzej Dziembowski

hDIS3 is a mainly nuclear, catalytic subunit of the human exosome complex, containing exonucleolytic (RNB) and endonucleolytic (PIN) active domains. Mutations in hDIS3 have been found in ∼10% of patients with multiple myeloma (MM). Here, we show that these mutations interfere with hDIS3 exonucleolytic activity. Yeast harboring corresponding mutations in DIS3 show growth inhibition and changes i...

2016
W Michael Dismuke Mikael Klingeborn W Daniel Stamer

Exosomes are emerging as important mediators of cell-matrix interactions by means of specific adhesion proteins. Changes in the tissue-specific exosomal protein expression may underlie pathological conditions whereby extracellular matrix turnover and homeostasis is disrupted. Ocular hypertension due to extracellular matrix accumulation in the trabecular meshwork is a hallmark of glucocorticoid-...

Journal: :Biochemical Society transactions 2014
Phil Mitchell

The exosome ribonuclease complex functions in both the limited trimming of the 3'-ends of nuclear substrates during RNA processing events and the complete destruction of nuclear and cytoplasmic RNAs. The two RNases of the eukaryotic exosome, Rrp44 (rRNA-processing protein 44) and Rrp6, are bound at either end of a catalytically inert cylindrical core. RNA substrates are threaded through the int...

2014
Jan Van Deun Pieter Mestdagh Raija Sormunen Veronique Cocquyt Karim Vermaelen Jo Vandesompele Marc Bracke Olivier De Wever An Hendrix

Despite an enormous interest in the role of extracellular vesicles, including exosomes, in cancer and their use as biomarkers for diagnosis, prognosis, drug response and recurrence, there is no consensus on dependable isolation protocols. We provide a comparative evaluation of 4 exosome isolation protocols for their usability, yield and purity, and their impact on downstream omics approaches fo...

Journal: :Cell 2011
Uttiya Basu Fei-Long Meng Celia Keim Veronika Grinstein Evangelos Pefanis Jennifer Eccleston Tingting Zhang Darienne Myers Caitlyn R. Wasserman Duane R. Wesemann Kurt Januszyk Richard I. Gregory Haiteng Deng Christopher D. Lima Frederick W. Alt

Activation-induced cytidine deaminase (AID) initiates immunoglobulin (Ig) heavy-chain (IgH) class switch recombination (CSR) and Ig variable region somatic hypermutation (SHM) in B lymphocytes by deaminating cytidines on template and nontemplate strands of transcribed DNA substrates. However, the mechanism of AID access to the template DNA strand, particularly when hybridized to a nascent RNA t...

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