objective(s):p in vitro chemosensitivity and resistance assays (csras) are a promising tool for personalized treatment of glioblastoma multiform (gbm). these assays require a minimum of 1 to 2 g of tumor specimen for testing, but this amount is not always accessible. we aimed to assess the feasibility and validity of utilizing stereotactic biopsies of gbm in csras. materials and methods: single...

Use of light-emitting diodes (LEDs) in strawberry vitro multiplication The refinement plant tissue culture techniques is great importance for optimizing rates and improving the quality micropropagated changes, either or latest models light sources light..The objective this work was to evaluate effect type on Tudla cultivar. Strawberry sprouts were grown 30 days MS medium plus 100 mg L-1 myo-ino...

PURPOSE Simple limbal epithelial transplantation (SLET) and cultivated limbal epithelial transplantation (CLET) are proven clinical techniques for treating limbal stem cell deficiency (LSCD). However, the ideal size and number of the limbal explants required for transplantation has not been clearly elucidated. This in vitro study aimed to determine the optimal limbal explant size required for c...

Tissue culture applications help mass production for many plant species, especially horticultural plants, ornamental medicinal and aromatic plants. Therefore, optimization of techniques is very important. In this study, the effects different Murashige Skoog (MS) salt levels (25%-200%) on in vitro Staurogyne repens (Nees) Kuntze were investigated. The shoot tip was used as explant. Different MS ...

four separate experiments were conducted to study the effects of factors controlling the establishment of explants from two walnut genotypes z60 and z63 (damavand). factors were explant type, sampling time, explant orientation on the growth medium and antioxidants type. the explants were collected from the collection garden of tooyserkan walnuts research station. four explant types (apical meri...

Shoot organogenesis and plant regeneration in Sinningia speciosa were improved using ethylene inhibitors. The leaf explants were cultured on initial shoot regeneration media (MS media with BAP at 2 mg/L + NAA at 0.1 mg/L) supplemented with different concentrations of aminoethoxyvinylglycine (AVG), cobalt chloride (CoCl₂), and silver thiosulphate (STS). The addition of AVG, CoCl₂, and STS signif...

OBJECTIVES Left ventricular assist device (LVAD) explantation and exchange is a relatively infrequent but potentially complex procedure. Patients requiring such procedures have multisystem suboptimal physiological reserve due to end-stage heart failure and are prone to complications. Less-invasive procedures are believed to facilitate postoperative recovery and early mobilization. We describe a...