BACKGROUND The hepatitis C virus (HCV) Alternate Reading Frame Protein (ARFP or F protein) presents a double-frame shift product of the HCV core gene. We and others have previously reported that the specific antibodies against the F protein could be raised in the sera of HCV chronically infected patients. However, the specific CD4(+) T cell responses against the F protein during HCV infection a...

Table 1. Effect of methionine addition to a protein free diet on cell composition Tissue composition was measured as described in the text on rats fed for 30 days on a protein-free diet supplemented or not with 0.3% methionine and in control rats at the beginning of the experiment of 150g on a normal diet (A) or fed for 30 days on a complete diet with 18% protein (B). Values represent the means...

Bitterness is a considerable limiting factor for the application of bioactive peptides in food industry. The objective this study was to compare level bitterness milk-protein-derived using an electronic tongue (E-tongue). Liquid milk protein concentrate (LMPC) prepared from ultra-heat-treated skimmed cow’s milk. It initially hydrolyzed with different concentrations trypsin, namely, 0.008 g·L−1,...

BACKGROUND: Overuse of fertilizers can lead to groundwater contamination, depletion of soil microorganisms and beneficial insects, increased crop susceptibility to diseases, acidification or alkalization of soil, damage to soil structure, reduced organic matter and soil fertility. Therefore, for achieve sustainable agricultural, it is necessary to reduce the consumption of conv...

in order to evaluation of the relationship among protein markers and traits such as proline content variation, quantum efficiency of photosystem ii and field viability index, 20 genotypes of barely were studied in a randomized complete block design in the greenhouse experiment. plants were transferred to springhouse at the four leaf stage and were located at the temperature of 4°c for three wee...

The Hendra virus fusion (F) protein is synthesized as a precursor protein, F(0), which is proteolytically processed to the mature form, F(1) + F(2). Unlike the case for the majority of paramyxovirus F proteins, the processing event is furin independent, does not require the addition of exogenous proteases, is not affected by reductions in intracellular Ca(2+), and is strongly affected by condit...

For paramyxoviruses, two viral glycoproteins are key to the entry process: an attachment protein (HN, H, or G) and the fusion protein (F). The F protein folds to a metastable state that can be triggered to undergo large conformational rearrangements to a fusogenic intermediate and a more stable postfusion state. The triggering mechanism that controls paramyxovirus fusion has not been elucidated...