BACKGROUND The aim of present work was to assess the concentration levels as well as vertical distribution of indicator bacteria including total coliform, fecal coliform, Pseudomonas aeruginosa, and Heterotrophic Plate Count (HPC) in the marine environment (seawater and coastal sediments) and evaluate the correlation between indicator bacteria and some physicochemical parameters of surface sedi...

We developed a chemiluminometric assay of beta-galactosidase in coliform bacteria, using a phenylgalactose-substituted 1,2-dioxetane derivative as a substrate. Permeabilization of cells is required to ensure the efficient cellular uptake of this compound. By this method, one coliform seeded in 100 ml of sterile water can be detected after a 6- to 9-h propagation phase followed by a 45-min enzym...

Contamination of surface waters with fecal bacteria from grazinglands is a component of nonpoint source agricultural pollution. Methods are needed to limit the movement of fecal bacteria from grazinglands into surface water. We used two experimental watersheds to test for impact of cattle on total coliform, E. coli, and enterococci bacteria numbers. Grazing cattle elevated these microbe numbers...

The current investigation was done to assess the bacterial load and detect the presence of indicator bacteria that acquire the spread of feacal contamination of water of river Ganga at Serampore, West Bengal, India. Total of 110 bacterial isolates, 25 feacal coliform, 50 Escherichia coli and Enterobacter sp were isolated from river water. The objective of this study also showed the antibiotic r...

A new radiometric method for the detection of coliform bacteria in water has been described. The method is based on the release of (14)CO(2) from [(14)C]lactose by bacteria suspended in growth medium and incubated at 37 C. The evolved (14)CO(2) is trapped by hyamine hydroxide and counted in a liquid scintillation spectrometer. The method permits the detection of 1 to 10 organisms within 6 h of ...

Continuous culture on mixed glucose-lactose or glucose-butyrate media inoculated with river water led to a population composed of a pseudomonad and a coliform. The glucose was used preferentially to the other carbon source, and the utilization of the secondary carbon source was greatly reduced at high growth rates. Significant amounts of acetate were excreted even though the cultures were limit...

Seawater and shellfish samples collected in the vicinity of a marine sewage outfall were examined for the incidence of antibiotic resistance among coliform and fecal coliform bacteria over a 2-year period. Seventy percent or more of these two groups of bacteria from both sources were resistant to one or more antibiotics. Forty-five percent of the isolates resistant to streptomycin or tetracycli...

Samples of drinking water from different sources in greater Cairo, Egypt, and bottled drinking water were tested for total coliform, fecal coliform, and coliphage populations. Of the 147 samples tested, 4 samples were positive for both total coliforms and coliphage, 65 samples were negative for total coliforms, fecal coliforms, and coliphage, and 78 samples were positive for coliphage and negat...

Survival of total and fecal coliform bacteria was measured in the 0 to 5, 5 to 15, and 15 to 30 cm soil depths at 1, 3, 7, 14, and 90 to 120 d after swine (Sus scrofa) wastewater application to riparian filterstrips in southern Georgia during each season of the year. Vegetative treatments evaluated were: (i) 20 m grass-10 m forest, (ii) 10 m grass-20 m forest, and (iii) 10 m grass-20 m maidenca...