Successfully developed in 1976, the continuous vitro culture of Plasmodium falciparum has many applications field malaria research. It become an important experimental model that directly uses a human pathogen responsible for high prevalence morbidity and mortality parts world is major source biological material immunological, biochemical, molecular, pharmacological studies. Until present, basi...

BACKGROUND Over a 16-year period, we conducted a clinical study of malaria acquired worldwide in adults from malaria-nonendemic countries, to determine risk factors for severe Plasmodium falciparum malaria. METHODS All patients with confirmed malaria who were managed by our unit from 1991 to 2006 were prospectively evaluated. Factors predicting disease severity according to (1) strict World H...

BACKGROUND Where P. vivax and P. falciparum occur in the same population, the peak burden of P. vivax infection and illness is often concentrated in younger age groups. Experiences from malaria therapy patients indicate that immunity is acquired faster to P. vivax than to P. falciparum challenge. There is however little prospective data on the comparative risk of infection and disease from both...

In this paper, a biosensors based on two-dimensional photonic crystal (2D PhC) waveguide including ring resonator is designed and simulated refractive index changes of red blood cells. The proposed biosensor structure consists an elliptical two linear waveguides containing silicon nitride rods in 2D rectangular lattice with circular rods. utilized to detect the stages Plasmodium falciparum cycl...

Severe falciparum malaria is common in tropics, patients usually present with any of the following manifestations or combination of cerebral manifestations, circulatory failure, liver failure, acute lung injury and acute kidney injury. Pulmonary manifestations in falciparum malaria usually include acute respiratory distress syndrome (ARDS) and acute lung injury (ALI)1. Here, we present two case...

Glucose stimulates the high-affinity processes of chloroquine and amodiaquin accumulation in owl monkey erythrocytes infected with a chloroquine-susceptible strain of Plasmodium falciparum. Although these erythrocytes have greater ability to accumulate amodiaquin than chloroquine, glucose has relatively less effect on amodiaquin accumulation than on chloroquine accumulation. In contrast to thes...

OBJECTIVE The present study aims to find out the mutational prevalence of Plasmodium falciparum chloroquine resistance transporter (Pfcrt) gene in Assam and Arunachal Pradesh, India. METHODS To fulfil the objective of the study, a total of 54 P. falciparum malaria positive samples were attempted for genotyping of Pfcrt gene using polymerase chain reaction (PCR) and direct DNA sequencing metho...

BACKGROUND Our study aimed at substantiating the recent claim of myocardial complications in severe malaria by experimentally inducing severe Plasmodium falciparum infection in a humanized mouse model employed as human surrogate. METHODS Twenty five humanized mice were inoculated with standard in vitro cultured P. falciparum and blood extracts collected from the inner cardiac muscles of infec...

Genetic manipulation has revolutionized research in the Apicomplexan parasite Plasmodium falciparum, the most important causative agent of malaria. However, to date no techniques have been established that allow modifications that are deleterious to blood-stage growth, such as the disruption of essential genes or the expression of dominant-negative transgenes. The recent establishment of a scre...