Various types of feeder cells have been adopted for the culture of human embryonic stem cells (hESCs) to improve their attachment and provide them with stemness-supporting factors. However, feeder cells differ in their capacity to support the growth of undifferentiated hESCs. Here, we compared the expression and secretion of four well-established regulators of hESC pluripotency and/or different...

Glycolysis is an essential component of cellular metabolism associated with pluripotent stem cells (PSCs). Two new papers, one by Gu et al. (2016) in this issue of Cell Stem Cell and one by Zhang et al. (2016) in Cell Reports, demonstrate that glycolytic flux is dynamically increased in human primed PSCs upon feeder-free cultivation or conversion into the naive state.

An optimum culture condition was established for our keratinocyte cell culture system from human adult skin using 3T3 feeder cells. Calcium ion (Ca(+)+ concentration was found to be critical and cells grew best at the Ca(+)+ concentration of 0.2 mM. Keratinocyte proliferation was promoted when 0.4 micrograms/ml hydrocortisone and 7 micrograms/ml insulin were added. However, epidermal growth fac...

embryonic stem cells (escs) are originally derived from the icm of blastocysts and are characterized by their ability to self-renew and their pluripotencies. only a few reports have been published on esc isolations and line establishment in animals, even fewer in horses. however, it is still important to isolate equine escs for animal biotechnology and therapeutic applications. in the present s...

: This article describes a good manufacturing practice (GMP)-compatible, feeder-free and serum-free method to produce large numbers of erythroid cells from human pluripotent stem cells (hPSCs), either embryonic or induced. This multistep protocol combines cytokines and small molecules to mimic and surpass the early stages of development. It produces, without any selection or sorting step, a pop...

KISCOi001-A is a healthy feeder-free and fully characterized human induced pluripotent stem (iPS) cell line cultured under xeno-free defined conditions. The generated from normal foreskin fibroblasts with non-integrating episomal plasmid vectors encoding OCT4, SOX2, KLF4, NANOG, LIN28, nontransforming L-MYC dominant negative p53. iPS cells are transgene-free their pluripotency confirmed by the ...