Abstract Leptomeningeal metastasis (LM), the spread of cancer into cerebrospinal fluid (CSF)-filled coverings brain and spinal cord, is a dismal complication leading to rapid neurologic disability death. While diverse array primary tumors may result in LM, recent work from our laboratory (Chi Y et al 2020 Science) demonstrates conserved transcriptional signature, independent tumor, suggesting t...

The effects of glucose extremes on vascular physiology and endothelial cell function have been examined across a range of time scales. Not unexpectedly, chronic glucose exposure induces long term tissue effects. Yet short term exposure can also impose lasting consequences. The persistence of vascular pathology after euglycemic restoration further suggests a glucose exposure memory. Slow turnove...

In both vertebrates and invertebrates, generating a functional appendage requires interactions between ectoderm-derived epithelia mesoderm-derived cells. To investigate such interactions, we used single-cell transcriptomics to generate temporal cell atlas of the Drosophila wing disc from two developmental time points. Using these data, visualized gene expression using multilayered model catalog...

in this study, we have investigated the proliferative response of human dermal fibroblasts isolated from normal and psoriatic individuals to different cytokines. we found that il-1, tnf (α) and egf are stimulatory whereas ifn « and 1 both showed inhibitory activity on fibroblast proliferation. il-6 a more recently described cytokine, showed no detectable effects on cell proliferation. investiga...

determination of blood compatibility is an important problem in blood contacting devices. in this study, two classes of materials including polyurethane (based on polyethylene glycol and poly tetrametylene oxide) and polyvinyl alcohol samples, with different hydrophilicity properties were synthesized and their physico-chemical properties were compared. water uptake ratio, ftir spectroscopy, and...

background one of the main challenges in skin flap surgery is tissue ischemia and following necrosis. the present study compares the effects of fibroblast growth factors 1 and 2 on increasing cutaneous vasculature, improving ischemia, and preventing distal necrosis in ischemic skin flaps in rat model. methods thirty rats were allocated into 3 groups (n=10) and 2×8 cm dorsal random-pattern skin ...

The rate of collection of embryo fibroblast single cells by an embryo fibroblast monlayer was realted to the H-2 haplotype of the fibroblast monolayer. The rate was highest for the H-2s strains and lowest for the H-2k strains with all other strains examined being intermediate. As opposed to monolayers prepared from the A and C3H background animals, monolayers from B10 background mice only demon...

PURPOSE To evaluate the effect of the anti-VEGF-A monoclonal antibody bevacizumab on primary human Tenon's capsule fibroblasts (HTFs) in an in vitro model of wound healing. METHODS Fibroblasts were cultured in RPMI media, and bevacizumab was administered at a concentration ranging from 0.25 to 12.5 mg/mL. Fibroblast viability and cell death were assessed using the MTT colorimetric assay, lact...