Fresh or frozen tissue samples will always be the best tissue source for the analysis of nucleic acids and proteins from tissues. However, their long-term storage is expensive and laborious. Much interest has therefore been focused on the question whether the almost infinite resources of formalin fixed and paraffin embedded tissue samples in the archives of pathology and histology departments c...

in this study 485 specimen of garra rufa (heckel, 1843) from 29 populations from six river systems and basins including tigris, karkheh, karun, persis (helleh and mond), hormuz and maharlu were collected by a 5mm mesh sized seine net, anesthetized in 1% clove oil solution, fixed in 10% formalin and transferred to the laboratory for further investigations. some 28 morphometric, 25 morphometric p...

Large repositories of well characterized RNAlater preserved samples and formalin-fixed, paraffin-embedded samples have been generated worldwide. However, the impact on the proteome of the preservation methods remain poorly described. Therefore, we analyzed the impact on the proteome of preserving samples in RNAlater, and by formalin-fixation, paraffin-embedding on human soft tissue, using direc...

In this article we describe a method for colorimetric detection of miRNA in the kidney through in situ hybridization with digoxigenin tagged microRNA probes. This protocol, originally developed by Kloosterman and colleagues for broad use with Exiqon miRNA probes(1), has been modified to overcome challenges inherent in miRNA analysis in kidney tissues. These include issues such as structure iden...

3,4-Methylenedioxymethamphetamine (MDMA or ecstasy) is a commonly consumed recreational drug. As is the case with most secondary amines, MDMA reacts with formaldehyde under acidic conditions to form tertiary amines. This reaction is likely to occur in formalin-fixed tissue. In formalin solutions, MDMA is methylated producing 3,4-methylenedioxy-N,N-dimethylamphetamine (MDDA). MDDA standard was s...

samples of formalin fixed gastrointestinal organs of three month ostrich chicks were submitted to the department of pathology, razi vaccine and serum research institute. histologically hepatitis and pancreatitis with large eosinophilic intra nuclear inclusion bodies were prominent in hepatic and pancreatic cells. these inclusions were suspected as adenovirus inclusion body hepatitis (ibh) in va...

Objective To evaluate the margins of resected specimen of oral squamous cell carcinoma (SCC) and to document the surgical margin (measured at the time of resection) and margins at the time of pathological examination (after immersion of the specimen in formalin). Methods Patients who were diagnosed and confirmed with squamous carcinoma of buccal mucosa were included in the study. Patients und...

To study the effects of surface materials of cells on the behavior of other neighboring cells in a crowded culture, confluent sheets of rat 3Y1 fibroblasts were fixed and then 3Y1 cells were seeded on to them. Among confluent sheets unfixed, fixed with formalin and fixed with ethanol and an empty plastic dish surface, the substrate activity to permit cell adhesion was compared. After confluent ...

Diffusion tensor microimaging at 16.4 T with 40µm isotropic voxels was to investigate anisotropic water diffusion in prostate tissue at spatial resolution approaching the cellular scale. Nine normal tissue and two cancer samples were collected from the peripheral zone of six formalin fixed radical prostatectomy specimens. Fibromuscular stromal tissue exhibited microscopic diffusion anisotropy (...