In this study, we cultured Chlorella vulgaris cells with a range of lipid contents, induced via nitrogen starvation, and characterized them via flow cytometry, with BODIPY 505/515 as a fluorescent lipid label, and liquid-state H NMR spectroscopy. In doing so, we demonstrate the utility of calibrating flow cytometric measurements of algal lipid content using triacylglyceride (TAG, also known as ...

In this study, we cultured Chlorella vulgaris cells with a range of lipid contents, induced via nitrogen starvation, and characterized them via flow cytometry, with BODIPY 505/515 as a fluorescent lipid label, and liquid-state 1H NMR spectroscopy. In doing so, we demonstrate the utility of calibrating flow cytometric measurements of algal lipid content using triacylglyceride (TAG, also known as...

Background: Recently, modification of T cells with chimeric antigen receptor (CAR) has been an attractive approach for adoptive immunotherapy of cancers. Typically, CARs contain a single-chain variable domain fragment (scFv). Most often, scfvs are derived from a monoclonal antibody of murine origin and may be a trigger for host immune system that leads to the T-cell clearance. Nanobody is a spe...

background: celiac disease (cd) is a small bowel enteropathy caused by permanent wheat gluten intolerance. one of the earliest signs of the mucosal immune activation in cd is an increase in the intestinal intraepithelial lymphocytes (iiels) count in the small intestinal epithelium. although most of those iiels express ??tcr, cd is characterized by an increase in the gdtcr + iiels. objective: th...

background: human peripheral blood nk cells constitutively express cd56 and cd16 antigens. peripheral blood nk cells seem to be strongly related with decidual nk cells, and may reflect the decidual nk cell functional status. there are varied reports concerning the relationship between nk cell cytotoxicity in women with recurrent spontaneous abortion. objective: to study nk activity in women wit...

dendrostellera lessertii (thymelaeaceae) is a toxic plant that grows in parts of iran. the anti-proliferative properties of its crude methanol extract and one of its active components, 3-hydrogenkwadaphnin (3-hk), have been established using several cancer cell lines. methods: in a further attempt to determine the mode of action, two groups of synchronously growing cells were treated with a sin...

objective(s): gastric cancer is the first and second leading cause of cancer related death in iranian men and women, respectively. gastric cancer management is based on the surgery, radiotherapy and chemotherapy. in the present study, for the first time, the beneficial effect of saffron (crocus sativus l.) aqueous extract (sae) on the 1-methyl-3-nitro-1-nitrosoguanidine (mnng)-induced gastric c...

background: bernard-soulier syndrome is a rare inherited bleeding disease caused by quantitative or qualitative defect of gpib/ix/v, a platelet complex that binds the von willebrand factor. the expression of gpib-ix-v complex can be evaluated by flow cytometry and confirmed by the absence of ristocetin-induced platelet aggregation in platelet-rich plasma. the main aim of the present study was t...

background: activated normal platelets undergo many biochemical and morphological changes, some of which are apoptotic. platelet derived microparticles and shrinked platelets as hallmark of platelet activation and apoptosis disperse surfaces containing procoagulant activity around injured vessels and tissues. this study was conducted to determine microparticles formation and platelet shrinkage ...

background: the aim of this study was to detect the prognostic significance of survivin level and the expression of total p53 in acute lymphoblastic leukemia (all) and its correlation to patients' outcome. methods: sixty two children newly diagnosed with acute lymphoblastic leukemia were treated with chemotherapy and followed up for 2 years or until death. twenty apparently healthy volunteers w...