An enzymatic fluorometric assay for pyridoxal with pyridoxal dehydrogenase was developed. The detection limit was about 10 pmol: the calibration curve of pyridoxal showed high linearity (r=0.993). The values obtained by this method correlated well with those by the HPLC method. The enzyme had a high specificity for pyridoxal, and thus animal samples could be directly analyzed without separation...

The GenomEra MRSA/SA assay (Abacus Diagnostica, Turku, Finland) is the first commercial homogeneous PCR assay using thermally stable, intrinsically fluorescent time-resolved fluorometric (TRF) labels resistant to autofluorescence and other background effects. This fully automated closed tube PCR assay simultaneously detects Staphylococcus aureus specific DNA and the mecA gene within 50 min. It ...

A sensitive fluorometric assay for the quantification of phosphite has been developed. The assay uses the enzymatic oxidation of phosphite to phosphate by a recombinant phosphite dehydrogenase with NAD(+) as cosubstrate to produce the highly fluorescent reaction product resorufin. The optimized assay can be carried out in a 96-well microtiter plate format for high-throughput screening purposes ...

The popular Mattingly [J. Gun. Pathol. 15, 374 (1962)] fluorometric assay for plasma cortisol is shown to give rise to falsely high values when performed with the recommended optical arrangement. By exciting at 470 nm, however, results are obtained that correlate well (r = 0.972) with those of competitive protein binding. The source of the interference is not identified, but it is related to bo...

A fllwrometric amino acid analyzer using fllmresramine for the assay of the full array of natural amino ac<ids including prolinc on a single column is reported. The proline determination was carried out by specific introduction of a solution of ,\‘chlorosl~ccinimide into the flow system. Single rolumn fluorometric amino acid analysis was curried out in a significantly shorter time and with a se...