The present investigation represents the effect of freeze drying on some properties as acid and bile tolerance of Streptococcus thermophilus MTCC 1938 culture isolated from dairy products. The cell paste obtained from milk based medium was freeze dried with a pressure of 50-100 mtorr for 24h at -40°C. Acid and bile tolerance test exhibited 3.8-4.9 and 3.2-3.8 log counts reduction after freeze d...

BACKGROUND We recently showed that freeze-dried cells stored for 3 years at room temperature can direct embryonic development following cloning. However, viability, as evaluated by membrane integrity of the cells after freeze-drying, was very low; and it was mainly the DNA integrity that was preserved. In the present study, we improved the cells' viability and functionality after freeze-drying....

Mouse spermatozoa can be freeze dried without losing genetic integrity and reproductive potential. However, it is not known if freeze-dried mouse cells similarly maintain their genetic integrity and developmental potential following nuclear transfer. Here, we investigated the developmental capacity and embryonic stem (ES) cell derivation of reconstructed oocytes by nuclear transfer using freeze...

colleagues [1] on fresh frozen plasma (FFP) in massive bleedings. We fully agree with their conclusion when they say that we need immediately available universal donor AB plasma. Hemorrhagic shock is the primary cause of avoidable mortality in combatants. Data from the recent US army war experience show clearly that in cases of massive transfusion the transfusion policy strongly impacts mortali...

a Laboratory of Biophysics and Wageningen NMR Centre, Department of Agrotechnology & Food Sciences, Wageningen University, Dreijenlaan 3, P.O. Box 8128, NL-6700 ET Wageningen, The Netherlands b Unilever Research and Development Vlaardingen, Olivier van Noortlaan, P.O. Box 114, NL-3130 AC Vlaardingen, The Netherlands c Quantitative Imaging Group, Department of Imaging Science & Technology, Delft...

Concentration of factor VIII from fresh plasma by cryoprecipitation remains the basis for preparation of products used to treat haemophilia A. This paper describes the preparation of a factor VIII concentrate from small plasma pools in transfusion centres with drying facilities. The dried concentrate from one litre of plasma dissolves very well in 50 or 100 ml of distilled water and contains ar...

Response surface methodology (RSM) along with central composite design (CCD) was applied to optimize the freeze drying conditions for purified pectinase from mango (Mangifera indica cv. Chokanan) peel. The effect of pectinase content (-2.66, 62.66 mg/mL), Arabic gum (-1.21, 10.21%, w/v), and maltodextrin (0.73, 7.26%, w/v) as independent variables on activity, yield, and storage stability of fr...

Freeze-drying for electron microscopy is one of the most successful methods for avoiding specimen distortion caused by drying of liquid solutions in air. It has been calculated that the surface forces of receding droplets subject small specimens to pressures of the order of tons per square inch (1). In freeze-drying, an aqueous solution of the specimen is rapidly frozen and the ice allowed to s...

Cat’s claw (Uncaria tomentosa) is a medicinal plant from the Amazon River basin that is widely used for inflammatory disorders and was previously described as an inhibitor of NF-kB. Cat’s claw was prepared as a decoction (water extraction) of micropulverized bark with and without concentration by freeze-drying. Murine macrophages (RAW 264.7 cells) were used in cytotoxicity assays (trypan blue e...

In this study, TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl)-oxidized nanocellulose superabsorbents are prepared using three different drying techniques: freeze-dried, and oven-dried at low high temperatures. The benefits investigated by evaluating the superabsorbent structure, composition application rate. absorption performance of is affected concentration type salts in soil water extracts. Ov...