ethanolic crude extract of i. imbricata lindl. (iridaceae) was subjected to column chromatography on silica gel with varying portions of meoh: chcl3. nigicin (irisolone) was isolated and its identification carried out by ir, uv, ms, 1-d and 2-d nmr spectroscopy.

Murine T cell replacing factor (TRF) was purified from a cellfree supernatant of a T cell hybridoma (B151K12) that constitutively produces TRF. Two assay systems for TRF activity were employed: 1) induction of anti-DNP IgG PFC responses in cultures of splenic B cells from DNP-KLH-primed BALB/c mice, and 2) induction of IgM PFC in chronic B cell leukemic cells (BCL1). The purification scheme con...

Rabbit testis contains a single molecular form of proacrosin which could be extracted at acid pH and purified 145-fold by Sephadex G-100, SE-Sephadex, and concanavalin A-Sepharose affinity column chromatography. The purified proacrosin was judged to be homogeneous on the basis of chromatographic analyses, disc and sodium dodecyl sulfate-polyacrylamide disc gel electrophoresis, and immunodiffusi...

A new antibiotic, aldecalmycin, has been discovered in the culture broth of Streptomyces sp. MJ147-72F6. Aldecalmycin was purified by solvent extraction, Diaion HP-20 chromatography, silica gel chromatography, Sephadex LH-20 chromatography, HPLC and centrifugal partition chromatography. The *H and 13C NMRspectra of aldecalmycin showed the presence of keto-enol tautomers. Aldecalmycin is equipot...

background and objectives: many members of the amaryllidaceae are regarded as toxic. the toxic constituents that occur in the whole family are referred to as the amaryllidaceae alkaloids. the main aim of this study was the identification of alkaloid compounds from galanthus transcaucasicus fomin, a medicinal plant from amaryllidaceae. methods: planar and column chromatography techniques were us...

A modern apparatus for performing flash chromatography using commercially available, prepacked silica cartridges has been developed. The key advantage of this system, when compared to traditional flash chromatography, is its use of commercially available silica cartridges, which obviates the need for students to handle silica gel. The apparatus has been tested for its ability to perform separat...

This article reviews the progress of a personal endeavour to develop chromatography as a quantitative procedure for the determination of reaction stoichiometries and equilibrium constants governing protein interactions. As well as affording insight into an aspect of chromatography with which many protein chemists are unfamiliar, it shows the way in which minor adaptations of conventional chroma...

Asphalt aging is one of the main causes asphalt pavement cracking, loosening and other issues. On a macro level, hardens becomes more brittle, while on micro chemical composition molecular weight distribution change. This very complicated physicochemical process. Gel permeation chromatography (GPC) powerful technical tool for understanding mechanism expressing GPC can be used to measure content...

Background: The chaperone activity of Mycobacterium tuberculosis Acr is an important function that helps to prevent misfolding of protein substrates inside the host, especially in conditions of hypoxia. Objectives: The aim of this study was to establish the correlation of structure and function of recombinant Acr proteins both before and after ge...

The separations of alcohols with hydrophilic and hydrophobic parts, and the separation of aromatic mixtures, are extremely important processes in gas and petroleum industries. Choosing an adsorbent for performing this separation is the most important part of the process. Silica gel is used as an adsorbent is various techniques such as pressure swing adsorption (PSA) and gas and liquid chromatog...