This paper describes a new design of standard agarose gel electrophoresis procedure for nucleic acids analysis. The electrophoresis was improved by using the real-time spectral analysis of the samples to increase its performance. A laser beam illuminated the analysed sample at wavelength with the highest absorption of gel components. Thus, DNA band is detected well before it may exits a gel pla...

1 The abbreviations used are: KDO, 3-Deoxy-D-manno-octulosonate; KDO 8-P, 3-DeoxyD-manno-octulosonate 8-phosphate; Tris, Tris-(hydroxymethyl)aminomethane; HEPES, N-[2Hydroxyethyl]piperazine-N’-[2-ethanesulfonic acid]; PNPase, purine nucleoside phosphorylase; EDTA, (ethylenedinitrilo)tetraacetic acid; 2D gel electrophoresis, two dimensional gel electrophoresis; SDS-PAGE, sodium dodecyl sulfate-p...

2D polyacrylamide gel electrophoresis has been the traditional workhorse of proteomics, allowing for the resolution of several thousand proteins in a single gel. Difference gel electrophoresis is an emerging technology that allows for accurate quantification with statistical confidence while controlling for nonbiologic variation, and also increases the dynamic range and sensitivity of tradition...