To avoid artefacts introduced by culturing cells for extended periods of time, it is crucial to use low-passage patient-derived tumour cells. The ability to enrich, isolate and assay sub-populations of cells that behave as cancer stem cells (CSCs) from these primary cell lines is essential before performing characterizations such as gene-expression profiling. We have isolated cells from gliobla...

Alterations of the PTEN gene occur in glioblastoma multiforme. To determine the frequency of PTEN alteration, 34 consecutive glioblastomas were studied in detail. Sequencing each of the nine exons amplified from tumor DNA revealed 11 mutations. Analysis of polymorphic markers within and surrounding the PTEN gene identified an additional four homozygous deletion mutations. Loss of heterozygosity...

We show a detailed magnetic resonance (MR) examination of two rare pericallosa lipomas with a fat suppression imaging sequence and proton MR spectroscopy. For comparison, subcutaneous fat and lipids in glioblastomas were examined with H MR spectroscopy in vivo and ex vivo. Fat suppression makes the identification of lipomas simple. It is suggested that H MR spectroscopy shows no differences in ...

Glioblastomas are the most aggressive fast-growing primary brain cancer which originate in glial cells of brain. Accurate identification malignant tumor and its sub-regions is still one challenging problems medical image segmentation. The Brain Tumor Segmentation Challenge (BraTS) has been a popular benchmark for automatic glioblastomas segmentation algorithms since initiation. In this year, Br...

BACKGROUND Prominence of glycolysis in glioblastomas may be non-specific or a feature of oncogene-related subgroups (i.e. amplified EGFR, etc.). Relationships between amplified oncogenes and expressions of metabolic genes associated with glycolysis, directly or indirectly via pH, were therefore investigated. METHODS Using multiplex ligation-dependent probe amplification, copy numbers (CN) of ...

Hypothalamic growth hormone (GH)-releasing hormone (GHRH) stimulates the synthesis and release of GH from the pituitary gland. GHRH and its mRNA are also found in human cancers of the breast, ovary, prostate, lung, and other tumors, suggesting that GHRH is also a tumor growth factor. Various studies show that GHRH antagonists have antiproliferative effects in many tumor models; however, gliobla...

Loss of function of the p53 tumor suppressor gene due to mutation occurs early in astrocytoma tumorigenesis in about 30-40% of cases. This is believed to confer a growth advantage to the cells, allowing them to clonally expand due to loss of the p53-controlled G1 checkpoint and apoptosis. Genetic instability due to the impaired ability of p53 to mediate DNA damage repair further facilitates the...