results results of the current study showed that the minimum inhibitory concentration value of cinnamaldehyde against m. tuberculosis was 200 µg/ml. moreover, rt-pcr data showed that a total of 25 genes were regulated by the cinnamaldehyde. of these, 12 genes were up-regulated, and 13 genes were down-regulated. conclusions cinnamaldehyde is a pattern to expand the new anti-tb drugs, because the...

BACKGROUND Drug-resistant tuberculosis (DR-TB) is considered a real threat to the achievement of TB control. Testing of mycobacterial culture and testing of drug susceptibility (DST) capacity are limited in resource-poor countries, therefore inadequate treatment may occur, favouring resistance development. We evaluated the molecular assay GenoType MTBDRplus (Hain Lifescience, Germany) in order ...

macrophages and t-lymphocytes are involved in immune response to mycobacterium tuberculosis. macrophage produces interleukin (il)-1 as an inflammatory mediator. il-1 receptor antagonist (il1-ra) is a natural antagonist of il-1 receptors. in this study we aimed to examine the possible association between the variable number of tandem repeats (vntr) of the il-1 receptor antagonist (il1rn) gene an...

Background: Drug resistance levels and patterns among Mycobacterium tuberculosis isolates from newly diagnosed and previously treated tuberculosis patients in Mbarara Uganda were investigated. Methods: We enrolled, consecutively; all newly diagnosed and previously treated smearpositive TB patients aged ≥ 18 years. Isolates were tested for drug resistance against rifampicin and isoniazid using t...

The testis brain RNA-binding protein (TB-RBP/translin) is a DNA- and RNA-binding protein with multiple functions. As an RNA-binding protein, TB-RBP binds to conserved sequence elements often present in the 3' untranslated regions (UTRs) of specific mRNAs modulating their translation and transport. To identify additional mRNA targets of TB-RBP, immunoprecipitation and reverse transcription-polym...

BACKGROUND Infection with Mycobacterium tuberculosis (Mtb) results in different clinical outcomes ranging from asymptomatic containment to rapidly progressing tuberculosis (TB). The mechanisms controlling TB progression in immunologically-competent hosts remain unclear. METHODOLOGY/PRINCIPAL FINDINGS To address these mechanisms, we analyzed TB progression in a panel of genetically heterogeneo...

To improve multidrug-resistant tuberculosis (MDR-TB) detection, we successfully introduced the rpoB gene mutation line probe assay into the national laboratory in Latvia, a country with epidemic MDR-TB. The assay detected rifampin resistance with 91% sensitivity and 96% specificity within 1 to 5 days (vs. 12-47 days for BACTEC).

BACKGROUND A number of observational studies have been conducted to investigate the association of IL-10 gene polymorphisms with tuberculosis (TB) susceptibility. However, the results of different studies were inconsistent. The aim of this study was to investigate the relationship between IL-10 -1082G/A, -819T/C, and -592A/C polymorphisms and TB risk by meta-analysis. METHODS A literature sea...