The aim of this analysis is to assess the genetic diversity in the black-coated Asturcón pony population using genealogical and molecular methods in order to ascertain the possible impact of the inclusion of the bay Asturcón individuals on the breed's recovery programme. Pedigree information registered in the studbook of the black-coated Asturcón (including a total of 1080 individuals) was anal...

Purpose Vertical cup-disc ratio (VCDR) is used as a clinical assessment measure to identify and monitor glaucomatous damage to the optic nerve. Previous genetic studies conducted in European and Asian populations have identified many loci associated with VCDR. The genetic factors in other ethnic populations, such as Latino, influencing VCDR remain to be determined. Here, we describe the first g...

The relative contributions of transmission and reactivation of latent infection to TB cases observed clinically has been reported in many situations, but always with some uncertainty. Genotyped data from TB organisms obtained from patients have been used as the basis for heuristic distinctions between circulating (clustered strains) and reactivated infections (unclustered strains). Naïve method...

The Carassius auratus complex in natural populations includes diploid triploid and polyploidy individuals. Diploid individuals belong to the species Carassius auratus whereas triploid and polyploidy individuals are from the subspecies Carassius auratus gibelio. Triploid individuals are all female and reproduce clonally by gynogenesis. Therefore the Carassius auratus complex is an ideal system f...

Facing an alarming continuing decline of wild sea cucumber resources, management strategies were developed over the past three decades to sustainably promote development, maintenance, or regeneration fisheries. In New Caledonia (South Pacific), dedicated efforts via restocking and ranching programs implemented cope with overharvesting sandfish Holothuria scabra recent loss known populations. or...

This study was conducted to assess the potential association between leptin (LEP) gene polymorphisms and type 2 diabetes mellitus (T2DM) in Iraqi patients. Genomic DNA extracted from 120 diabetic subjects 100 controls. Three specific PCR fragments were designed flank three highly frequent single nucleotide polymorphism (SNP)s within LEP, rs11761556, rs12706832 rs2167270. The amplified loci geno...

Long-term immunity to coronaviruses likely stems from T cell activity. We present here a novel approach for the selection of immunoprevalent SARS-CoV-2-derived epitopes using an in silico cohort HLA-genotyped individuals with different ethnicities. Nine 30-mer peptides derived four major structural proteins SARS-CoV-2 were selected and included peptide vaccine candidate recapitulate broad virus...

Next-generation massively parallel sequencing technologies provide ultrahigh throughput at two orders of magnitude lower unit cost than capillary Sanger sequencing technology. One of the key applications of next-generation sequencing is studying genetic variation between individuals using whole-genome or target region resequencing. Here, we have developed a consensus-calling and SNP-detection m...

The effects of allelic variants of CYP2C9 (CYP2C9*2 and CYP2C9*3) on lornoxicam 5'-hydroxylation were studied using the corresponding variant protein expressed in baculovirus-infected insect cells and human liver microsomes of known genotypes of CYP2C9. The results of the baculovirus expression system showed that CYP2C9.3 gives higher K(m) and lower V(max) values for lornoxicam 5'-hydroxylation...

The pattern and nature of linkage disequilibrium in the human genome is being studied and catalogued as part of the International HapMap Project [:2003 Nature 426:789-796]. A key goal of the HapMap Project is to enable identification of tag single nucleotide polymorphisms (SNPs) that capture a substantial portion of common human genetic variability while requiring only a small fraction of SNPs ...