-This paper proposes an improved two-step classification scheme for Giemsa-stained human chromosomes using a hierarchical multilayer neural network with an error back-propagation training algorithm. In the first step, the Group Classifier (GC), a two-layer neural network, classifies chromosomes into seven groups based on their morphological features, such as relative length, relative area, and ...

A 66-year-old man presented with a white blood cell count of 62.63 10 /L. Peripheral blood (PB) smear showed 80% smallto medium-sized atypical lymphoid cells with irregular to cleaved (panel A; original magnification 3500, Wright-Giemsa stain) or binucleated nuclear contours, and slightly condensed chromatin (panel B; original magnification 31000, Wright-Giemsa stain). Bone marrow (BM) aspirate...

BACKGROUND Accuracy of diagnosis is the core for malaria control. Although microscopy is the gold standard in malaria diagnosis, its reliability is largely dependent on user skill. We compared performance of Cyscope fluorescence microscope with the Giemsa stained light microscopy for the diagnosis of malaria among pregnant women at Medani Hospital in Central Sudan. The area is characterized by ...

Malaria is a responsible for approximately 600 thousand deaths worldwide every year. Appropriate and timely treatment of malaria can prevent deaths but is dependent on accurate and rapid diagnosis of the infection. Currently, microscopic examination of the Giemsa stained blood smears is the method of choice for diagnosing malaria. Although it has limited sensitivity and specificity in field con...

BACKGROUND This study was to determine the sensitivity, specificity, positive predictive value (PPV), negative predictive values (NPV) and agreement between two methods of the stained gastric imprint cytology smears and stained gastric specimen biopsy mucosal methods for detection of H. pylori. METHODS Air-dried imprint smears of gastric biopsies from 330 patients were stained by the Grunwald...

Archived tissues from affected yellow perch Perca flavescens, as well as fresh submissions of juvenile yellow perch, walleye, fathead minnows, golden shiners and smallmouth bass cultured in the same pond or from a shared water source were examined for the presence of Myxobolus neurophilus. Archived tissues were sectioned and stained with hematoxylin and eosin or with Giemsa, revealing myxozoan ...

The ring-stage survival assay (RSA) is a powerful tool for phenotyping artemisinin-resistant Plasmodium falciparum but requires experienced microscopists to count viable parasites among 10,000 erythrocytes in Giemsa-stained thin blood smears. Here we describe a rapid flow cytometric assay that accurately counts viable parasites among 250,000 erythrocytes in suspension. This method performs as w...

Improved techniques consisting of glutaraldehyde fixation, Giemsa staining, and colony counter enumeration of foci in viral infectivity assays were compared to microscope scanning and lantern slide projection methods. These techniques broadened the scope of application to potentially hazardous viruses and increased the efficiency of assaying.

The karyological features of Akis subtricostata was determined for the first time with conventional and silver nitrate staining. diploid number 2n=16 meioformula 7+neoXY represents a deviation from modal karyotype Coleoptera. pericentromeric heterochromatin detected both Giemsa In addition to determining single possible NOR on prophase I nuclei, AgNO3 revealed that several telomeric regions mit...