Recent studies suggest that lead induces oxidative stress in various tissues. Captopril ([2S]-1-[3-mercapto-2-methylpropionyl]-L-proline), an angiotensin-converting enzyme inhibitor, is a well-known antihypertensive agent and is also believed to function as an antioxidant. In the present study the antioxidant effect of captopril on lead-induced oxidative stress was studied in Fischer 344 rats. ...

Glutathione dehydrogenase (EC 1.8.5.1) was partially purified from pea shoots. The pH optimum was 7.6. The K(m) values for GSH and dehydroascorbate were 4.4 and 0.44 millimolar, respectively. The enzyme was inhibited by iodoacetate and CuSO(4) but not significantly by ZnCl(2) or NaN(3). Part of the total enzyme activity was associated with isolated chloroplasts.Illuminated ruptured chloroplasts...

The reduced glutathione of normal and glucose-6-P dehydrogenase-deficient human erythrocytes was oxidized by subjecting the cells to HzOz diffusion or to treatment with methyl phenylazoformate. A portion of the oxidized glutathione thus formed disappeared from the erythrocytes and could be recovered from the medium when the normal cells were incubated in the absence of glucose or when glucose6-...

The objective of this study was to evaluate the concentrations total (T-GSH), oxidised (GSSG) and reduced (GSH) glutathione in blood dairy cows, assess relationships phase number lactation, milk production, body condition score (BCS) selected biochemical parameters on its concentrations. We analysed 79 samples whole from Holstein cows. concentration assessed by spectrophotometric enzymatic meth...

Acylfulvenes (AFs) are a class of antitumor agents with favorable cytotoxic selectivity profiles compared to their natural product precursor, illudin S. Like many alkylating agents, illudin S and AFs readily react with thiol-containing small molecules such as cysteine, glutathione and cysteine-containing peptides; reduced cellular glutathione levels can affect illudin S toxicity. Glutathione re...

BACKGROUND Protein Disulfide Isomerase (PDI) in the endoplasmic reticulum of all cells catalyzes the rearrangement of disulfide bridges during folding of membrane and secreted proteins. As PDI is also known to bind various molecules including hormones such as estradiol and thyroxin, we considered the hypothesis that adverse effects of endocrine-disrupter compounds (EDC) could be mediated throug...

Ethanol exposure during pregnancy elicits profound detrimental developmental and behavioral effects such as reduced levels of insulin-like growth factor-1 (IGF-1) in the fetus. However, few reports have addressed its impact on postpartum dams. This study was designed to examine the influence of gestational ethanol exposure on postpartum maternal organ oxidative damage and IGF-1 level. Pregnant ...

Colon cancer (CC) is accepted as the third type of that causes death in world. Oxidative stress and low glutathione peroxidase (GPx) activity can cause CC. This study aims to show whether GPx Oxidized (GSSG)/ reduced (GSH) levels, which are considered oxidative markers, effective etiopathogenesis Erythrocyte isolation was performed 3 ml blood sample taken from volunteers aged 18-75 years. Hemog...

Methylenedianiline (DAPM) initially injures epithelial cells of major bile ducts, which is followed by cholestasis, cholangitis, and hepatocellular damage. This pattern of biliary injury resembles that produced by alpha-naphthylisothiocyanate (ANIT), a classic bile duct toxicant. Our goal was to determine whether prior depletion of hepatic total glutathione (GSx), a condition reported to protec...

OBJECTIVE This study aimed to evaluate the protective effect of kaempferol against myocardial ischemia/reperfusion (I/R) injury in rats. METHOD Left ventricular developed pressure (LVDP) and its maximum up/down rate (±dp/dt max) were recorded as myocardial function. Infarct size was detected with 2,3,5-triphenyltetrazolium chloride staining. Cardiomyocyte apoptosis was determined using termin...