A radioimmunoassay for glyceollin I, the major phytoalexin produced by soybean (Glycine max [L.] Merr.), has been developed. Antibodies were raised in rabbits against a glyceollin I-bovine serum albumin conjugate. The antisera were used to establish a radioimmunoassay for glyceollin I using [(125)I]glyceollin I as the tracer. A logit plot of a standard concentration series yielded a straight li...

A novel actinomycete, strain S10(T), was isolated from rhizosphere soil of wild Vitis vinifera in Thinghir, Ouarzazate Province, Southern Morocco. The taxonomic status of this strain was established using a polyphasic approach. Strain S10(T) had white-grey aerial mycelium with long, spiral spore chains bearing smooth surfaced spores and produced a yellow diffusible pigment. Chemotaxonomic analy...

All animals, except for the placental mammals, conjugate their bile acids exclusively with taurine. However, in certain of the placental mammals, glycine conjugates are also found. The basis for the appearance of glycine conjugation among the placental mammals was investigated. The reaction of choloyl-CoA with glycine and taurine, as catalysed by the soluble fraction from guinea-pig liver, had ...

In this study, 130 uniform ‘Flame Seedless’ grape trees were selected for and subjected to the same cultural practices. The sprayed three times, before flowering, during full bloom, weeks later with following treatments: control (water only), 250, 500, 750 ppm glycine; 50, 100, 150 folic acid (FA); 2%, 4%, 6% leaf moringa aqueous extract (MLAE); their combinations. High-performance liquid chrom...

Effects of oral administration of NC-1100 on the metabolism of neuro active amino acids in rat brain were studied using stroke-prone spontaneously hypertensive (SHR-SP) and Wistar Kyoto rats. The repeated administration of NC-1100 induced a significant increase of r-aminobutyric acid (GABA) content in the cerebellum and medulla oblongata of SHR-SP. The decrease of aspartic acid contents in the ...

Mortality associated with endotoxin shock is likely mediated by Kupffer cells, alveolar macrophages, and circulating neutrophils. Acute dietary glycine prevents mortality and blunts increases in serum tumor necrosis factor-alpha (TNF-alpha) following endotoxin in rats. Furthermore, acute glycine blunts activation of Kupffer cells, alveolar macrophages, and neutrophils by activating a glycine-ga...

Glycine uptake governs glycine site occupancy at NMDA receptors of excitatory synapses. J. Neurophysiol. 80: 3336-3340, 1998. At central synapses occupation of glycine binding sites of N-methyl--aspartate receptors (NMDA-Rs) is a necessary prerequisite for the excitatory neurotransmitter glutamate to activate these receptors. There is conflicting evidence as to whether glycine binding sites nor...

Pizer, Lewis I. (University of Pennsylvania, Philadelphia). Glycine synthesis and metabolism in Escherichia coli. J. Bacteriol. 89:1145-1150. 1965.-A correlation was demonstrated between a nutritional requirement that can only be satisfied by glycine and the absence of the enzymatic capacity to interconvert l-serine and glycine. Serine synthesis from 3-phosphoglycerate was observed in the same ...

The catabolism of glycine in the isolated perfused rat liver was investigated by measuring the production of 14CO2 from [1-14C]- and [2-14C]glycine. Production of 14CO2 from [1-14C]glycine was maximal as the perfusate glycine concentration approached 10 mM and exhibited a maximal activity of 125 nmol of 14CO2 X g-1 X min-1 and an apparent Km of approximately 2 mM. Production of 14CO2 from [2-14...

Various strategic efforts to increase food production continue be made in an effort achieve self-sufficiency and security. Soybean is a national commodity support government programs with the aim of achieving self-sufficiency. The purpose this study was determine effect combination phosphate fertilizer rhizobium biofertilizer on growth soybean (Glycine max L) Grobogan variety. research conducte...