Two murine monoclonal antibodies, FMC 25 and AN 51, directed against distinct epitopes on the glycoprotein Ib complex, have been used to further define the mechanism of quinine/quinidine drug-dependent antibody interaction with platelets. FMC 25, directed against an epitope on glycoprotein IX, had no effect on platelet aggregation induced by collagen or adenosine diphosphate and little, if any,...

Density gradient methods have been used to characterize the sol and gel phases of sputum samples from two asthmatic patients, one extrinsic (AsEXT) and the other intrinsic (AsINT). The sputum samples were separated into sol and gel phases by ultracentrifugation (160,OOOg). Sol and gel were then separately subjected to density gradient ultracentrifugation (DGU) in CsBr to isolate mucus glycoprot...

1. A revised amino acid and carbohydrate composition of human Tamm-Horsfall glycoprotein is presented. 2. No significant differences were obtained in the amino acid composition of Tamm-Horsfall glycoprotein isolated from patients with cystic fibrosis. 3. The glycoprotein was shown to possess a high half-cystine content of 1 per 11-12 amino acid residues, which has been confirmed by performic ac...

Twenty-one monoclonal antibodies reactive with Junin virus structural proteins were produced and characterized. Using radioimmunoprecipitation and Western blot assays, 13 were found to react with the nucleoprotein, seven with the surface glycoprotein and one failed to react, but showed a fluorescent antibody staining pattern consistent with other glycoprotein-specific antibodies. In radioimmuno...

Mature glycoprotein spikes are inserted in the Lassa virus envelope and consist of the distal subunit GP-1, the transmembrane-spanning subunit GP-2, and the signal peptide, which originate from the precursor glycoprotein pre-GP-C by proteolytic processing. In this study, we analyzed the oligomeric structure of the viral surface glycoprotein. Chemical cross-linking studies of mature glycoprotein...

Heparin cofactor II is a plasma protein that inhibits thrombin rapidly in the presence of either heparin or dermatan sulfate. We have determined the effects of two glycosaminoglycan-binding proteins, i.e., histidine-rich glycoprotein and platelet factor 4, on these reactions. Inhibition of thrombin by heparin cofactor II and heparin was completely prevented by purified histidine-rich glycoprote...

The glycoprotein of pig gastric mucus has been isolated free of non-covalently bound protein as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and equilibrium density-gradient centrifugation. After reduction with 0.2 M-mercaptoethanol, protein was released from the glycoprotein, which consisted of a major 70000-mol.wt. component and a minor 60000-mol.wt. component. The 700...

Hepatitis C virus (HCV) contains two membrane-associated envelope glycoproteins, E1 and E2, which assemble as a heterodimer in the endoplasmic reticulum (ER). In this study, predictive algorithms and genetic analyses of deletion mutants and glycosylation site variants of the E1 glycoprotein were used to suggest that the glycoprotein can adopt two topologies in the ER membrane: the conventional ...

Overview of the Issue P-glycoprotein, encoded by the MDR1 (also known as the ABCB1 gene) plays an important role in the absorption, distribution, metabolism and excretion of many drugs. It is not surprising, then, that dysfunction of P-glycoprotein increases susceptibility to drug toxicity (antiparasitic agents, anti-diarrheal drugs, chemotherapeutic drugs, acepromazine, butorphanol and others)...

Myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) is an increasingly recognized disorder, particularly in children. Acute disseminated encephalomyelitis (ADEM) the most common presentation of paediatric MOGAD. Children with ADEM should always be evaluated for MOGAD, as appropriate and timely treatment may help improve long-term outcomes.