The principal components of a variety of mucous secretions have been found to be glycoproteins, many of mol.wt. 500000 or more (Gottschalk, 1972). We have isolated the glycoprotein from pig colonic mucus and examined its structure in order to compare it with that of the well-characterized glycoprotein of the gastric mucus from the same species (Snary & Allen, 1971 ; Allen, 1977). Mucosal scrapi...

Previous studies show that transient axonal glycoprotein-1, a ligand of amyloid precursor protein, increases the secretion of amyloid precursor protein intracellular domain and is involved in apoptosis in Alzheimer's disease. In this study, we examined the effects of transient axonal glycoprotein-1 on U251 glioma cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed t...

Identical twin sisters with Darier's disease were successfully treated with a combination of oral vitamins A and E after vitamin A alone in a higher dose did not yield a good response. Although synthetic retinoids are currently favoured drugs for disorders of keratinization, the usefulness of the synergistic action of these fat-soluble vitamins is recapitulated and the lack of side -effects whi...

The drug transporter P-glycoprotein (ABCB1) plays an important role in drug distribution and elimination, and when overexpressed it may confer multidrug resistance (MDR). P-glycoprotein is localized in the plasma membrane, especially within rafts and caveolae, characterized as detergent-resistant membranes (DRMs). This study investigated the effect of cholesterol depletion and repletion as well...

Louisianin A (1) was treated with phosphoryl chloride (POC13) at 120~ 125°C3) to obtain chloro derivative (2) in 86% yield. 2 was reduced by Zn-AcOH at 50°C4) to afford 3 (52% yield) and 4 (35% yield). Furthermore, under the same condition, 3 was dechlorinated to give 4 in 48% yield. Moreover, 4 was oxidized in the treatment of CrO3-pyridine5) to obtain louisianin C (5) in 75% yield. Finally, 5...

Cancer-associated galactosyltransferase acceptor (CAGA glycoprotein), a small glycoprotein purified from human malignant effusion that selectively kills transformed cells, was tritiated by reductive methylation in the presence of NaB(3)H(4). CAGA-glycoprotein-sensitive cells (baby-hamster kidney cells transformed by polyoma virus and chick-embryo fibroblasts infected with Ts68 temperature-sensi...