blue represent proteins in homologous families A and B, while green and black denote other families C and D. Reported BLAST similarities are depicted by curved edges (bottom). A and C coincide on a multi-domain protein (pink and green protein) which may induce false-transitivity – falsely clustering A with nonhomologous C due to local BLAST similarities of multi-domain protein (red edges). Corr...

A study to determine the protein digestibility-corrected amino acid score and protein efficiency ratio of nine different cooked Canadian pulse classes was conducted in support of the establishment of protein quality claims in Canada and the United States. Split green and yellow pea, whole green lentil, split red lentil, Kabuli chickpea, navy bean, pinto bean, light red kidney bean, and black be...

Apicomplexan parasites possess a plastid-like organelle called the apicoplast. Most proteins in the Toxoplasma gondii apicoplast are encoded in the nucleus and imported post-translationally. T. gondii apicoplast proteins often have a long N-terminal extension that directs the protein to the apicoplast. It can be modeled as a bipartite targeting sequence that contains a signal sequence and a pla...

Transient expression of proteins in plants has become a choice to facilitate recombinant protein production with its fast and easy application. On the other hand, host defensive mechanisms have been reported to reduce the efficiency of transient expression in plants. Hence, this study was designed to evaluate the effect of cap analog and Potato virus A helper component proteinase (PVA HC-Pro) o...

green architecture, or green design, is an approach to building that minimizes harmful effects on human health and the environment. the “green” architect or designer attempts to safeguard air, water, and earth by choosing eco-friendly building materials and construction practices. so, green architecture is building and structure design philosophy that aims at minimal use of non-renewable and/or...

Unmanned aerial vehicle-based multispectral imagery including five spectral bands (blue, green, red, red-edge, and near-infrared) for a rice field in the ripening stage was used to develop regression models predicting yield protein content select most suitable analysis method year-invariant model: partial least squares regression, ridge artificial neural network (ANN). The developed with six ve...

We inertially inject and study the contents of optically trappable aqueous nanodroplets (hydrosomes) emulsified in a perfluorinated matrix. A new piezoelectric actuated device for production of single hydrosomes on demand is introduced. Hydrosomes containing enhanced green fluorescent protein (EGFP) were injected, optically trapped, and held at the focus of an excitation laser in a confocal mic...

We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.