In this report, we describe the inactivation and site-specific light induction of plasmid expression using a photosensitive caging compound. Plasmids coding for luciferase were caged with 1-(4, 5-dimethoxy-2-nitrophenyl)diazoethane (DMNPE) and transfected into approximately 1-cm diameter sites of the skin of rats with particle bombardment. Skin sites transfected with caged plasmids did not expr...

Correlations between measures of attention and topographical abnormalities of evoked cortical potentials elicited during the Continuous Attention Test (CAT) were assessed in 50 schizophrenic patients, compared to 50 healthy subjects. For each group and for each CAT condition evoked responses consisted of six successive epochs (segments) of stable spatially configured potentials. Quantitative de...

We designed a new genetic tool to detect plasmid transfer under anaerobic and aerobic conditions. The system is based on the T7 RNA polymerase gene and a T7 promoter-driven oxygen-independent green fluorescent protein, evoglow, alone or in combination with red fluorescent protein DsRed. Constructs are available as plasmids and mini-mariner transposons.

Over the past decade, fluorescent proteins (FPs) have become ubiquitous tools in biological research. Yet, little is known about the natural function or evolution of this superfamily of proteins that originate from marine organisms. Using molecular phylogenetic analyses of 102 naturally occurring cyan fluorescent proteins, green fluorescent proteins, red fluorescent proteins, as well as the non...

There is an error in the legend for Fig 1, "Genomic location of ORF5 gene and Detection of ORF5 transcripts by RT-PCR and Northern blot from PCV2-infected cells," panel D. Please see the complete, correct Fig 1 legend here. article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the o...

A method is described that allows simultaneous measurement of two spectrally distinguishable green fluorescent protein (GFP) mutants with a confocal microscope. In contrast to previously described methods, neither UV excitation nor repetition of scans is required. Therefore the method is well-suited to the long-time observation of living cells in three-dimensional microscopy and time series rec...

Proteins are one the most widely studied biomolecules with diverse functions and applications. Aiming at overcoming current drawbacks of purification processes proteins, introduction ionic liquids (ILs) has been a hot topic research. ILs have applied in creation aqueous biphasic systems (IL-based ABS), solid-phase extractions through poly(ionic liquid)s (PILs) supported ionic-liquid phases (SIL...

Imaging of live cells has been revolutionized by genetically encoded fluorescent probes, most famously green and other fluorescent proteins, but also peptide tags that bind exogenous fluorophores1. We report here the development of protein reporters that generate fluorescence from otherwise dark molecules (fluorogens). Eight unique fluorogen activating proteins (FAPs) have been isolated by scre...