A novel, sensitive chemiluminescence (CL) immunoassay for Escherichia coli O157:H7 detection with signal dual-amplification using glucose oxidase (GOx) and laccase was investigated. The method was based on the characterization of a luminol-H2O2-laccase reaction. Compared with the horseradish peroxidase-based biosensor, laccase exhibited high catalytic activity in strong alkaline medium, which w...

Advanced Oxidation Processes (AOPs) have been employed to degrade biorefractory organic matters. This study investigates the combination of classical Fenton reaction with electrochemical oxidation, the electro-Fenton process, for the treatment of semi aerobic landfill leachate, collected from Pulau Burung Landfill Site (PBLS), Penang, Malaysia. The investigation has been carried out in batch re...

This research studies the effect regarding two plasma types, jet and plasma-activated water (PAW), on tooth root canal bacteria Enterococcus faecalis. The works with argon gas, it is generated by a power supply that operates at alternating high voltages in form of sinusoidal wave peak-to-peak value about 12 kV frequency 30 KHz its 200 watts. was utilized directly to treat indirectly activating ...

Autonomic dysfunction is recognized to contribute to cardiovascular consequences in obstructive sleep apnea/hypopnea syndrome (OSAHS) patients who present predominant cardiovascular sympathetic activity that persists during wakefulness. Here, we examined 1) the factors that influence sympathetic cardiac modulation in response to apneas/hypopneas; and 2) the influence of autonomic activity durin...

We investigated whether the stem cell that reconstitutes total erythropoiesis of a WBB6F1-W/Wv mouse differentiates into lymphoid lineage. The electrophoretic pattern of hemoglobin was used as a marker of the reconstitution; 3-phosphoglycerate kinase (PGK), an X chromosome-linked enzyme was used as a tool for estimating clonality. We injected 10(5) bone marrow cells of 5-FU treated C57BL/6-Pgk-...

Vitex doniana (West African plum or black plum) is a plant with varying phytoconstituents and biological activities across different countries. In this study, essential oils extracted from the leaves fruits of cultivated in Ghana were investigated for their antimicrobial antioxidant activities. The actions determined using hydrogen peroxide (H2O2), phosphomolybdenum, thiobarbituric acid reactiv...

Frutos de jabuticaba e pequi contêm compostos bioativos, como polifenóis, que apresentam ação antioxidante; entretanto, os mecanismos moleculares pelos quais esses efeitos são alcançados não estão totalmente elucidados. No presente estudo, células musculares camundongo (C2C12), induzidas ou por estresse oxidativo com peróxido hidrogênio (H2O2), foram tratadas extrato casca (JPE) aquoso (PAE) pa...

BACKGROUND In this report, the isorhamnetin 3-o-robinobioside and its original extract, the ethyl acetate extract, from Nitraria retusa leaves, were evaluated for their ability to induce antioxidant and antigenotoxic effects in human chronic myelogenous leukemia cell line. METHODS Nitraria retusa products properties were carried out by firstly evaluating their effects against lipid peroxidati...

BACKGROUND Lignin peroxidase (LiP) is the primary enzyme responsible for lignin degradation. In our previous work, in order to shorten the pretreatment time and increase the lignin degradation, we have pretreated the corn stalk (CS) using a combination of Aspergillus oryzae CGMCC 5992 solid-state fermentation and H2O2 treatment. RESULTS In the present study, one-factor-at-a-time design and re...