In cultured HEK, we observed that HD (0.3-1.0 mM)-induced DNA damage triggers DNA repair and apoptosis, which may be interdependent. We studied the effects of a general caspase inhibitor, Z-VADfmk (0.004 mM) and the Fas (CD95) receptor (induces apoptosis on Fas ligand binding) antibody on DNA damage and its repair in HD-exposed HEK. Both Z-VAD-fmk and the CD95 antibody reduced HD-induced DNA de...

Non-thermal plasmas are emerging as a novel tool for the treatment of living tissues for biological and medical purpose. In this study, we described the effect of 4 min dielectric barrier discharge (DBD) plasma on both T98G cancer and HEK normal cell lines in the presence of different concentrations of osmolytes. This treatment strategy shows a specific inhibitory effect of a 240 s plasma expos...

Activation of PKR (double-stranded-RNA-dependent protein kinase) by DNA plasmids decreases translation, and limits the amount of recombinant protein produced by transiently transfected HEK (human embryonic kidney)-293 cells. Co-expression with Ebola virus VP35 (virus protein 35), which blocked plasmid activation of PKR, substantially increased production of recombinant TPL-2 (tumour progression...

The immense volume of data generated by the suite of instruments on the Solar Dynamics Observatory (SDO) requires new tools for efficient identifying and accessing data that is most relevant for research. We have developed the Heliophysics Events Knowledgebase (HEK) to fill this need. The HEK system combines automated data mining using feature-detection methods and high-performance visualizatio...

DNA is a major cellular target of the vesicant chemical warfare agent sulfur mustard (SM, bis-(2-chloroethyl) sulfide). Others and we have proposed a possible role of apoptosis in SM vesication. Our results suggest that in SM-exposed human epidermal keratinocytes (HEK), DNA damage, DNA repair, and apoptosis may be interdependent. In HEK, SM causes cell death accompanied by caspase-3 activation ...

?nsano?lu, yeryüzünde var oldu?u andan itibaren sa?l???n? korumak ve maruz kald??? fizikî ruhî rahats?zl?klardan kurtulmak için sürekli bir aray?? mücadele içerisinde olmu?tur. Korunma, te?his tedavi ?eklinde kar??m?za ç?kan bu mücadele, geçmi?ten günümüze belli birtak?m bilgi, birikim deneyimin olu?mas?n? sa?lam??t?r. Zengin hazine niteli?i ta??yan tecrübe, halk hekimli?ini vücuda getirmi?tir....

Development of improved fluorescent voltage indicators is a key challenge in neuroscience, but progress has been hampered by the low throughput of patch-clamp characterization. We introduce a line of non-fluorescent HEK cells that stably express NaV 1.3 and KIR 2.1 and generate spontaneous electrical action potentials. These cells enable rapid, electrode-free screening of speed and sensitivity ...

The four highly conserved intracellular tyrosine residues of the P2X(1) ion channel were mutated into phenylalanine. Simultaneous electrophysiological and calcium measurements in transfected human embryonic kidney (HEK 293) cells indicated that Y362F and Y370F mutants were non-functional, despite their proper plasma membrane expression. The Y16F and Y363F mutants retained 2.2% and 26% of the wi...