In this study the membrane orientation of a tryptophan-flanked model peptide, WALP23, was determined by using peptides that were labeled at different positions along the sequence with the environmentally sensitive fluorescent label BADAN. The fluorescence properties, reflecting the local polarity, were used to determine the tilt and rotation angles of the peptide based on an ideal alpha-helix m...

The present study proposes an integrated prediction model for both shearing and ploughing constants the peripheral milling of Inconel 718 by using a preidentified mean normal friction coefficient. An equation is presented identification angle oblique cutting in milling. A simplified adopted obtaining shear strain tool given helix angle, radial rake honed edge radius. predicted from analytical M...

Using small-angle and wide-angle X-ray scattering techniques it has been possible to clarify the helix-to-sheet (cross) transition and the stacking process of the crosssheet of apomyoglobin as a model for amyloid. The present results indicate that the crossformation and the pleated sheet stacking start concurrently and that the stacking continues progressively after the saturation of the crossf...

We demonstrate a simple fabrication method of uniform-lying-helix (ULH) cholesteric liquid crystal (ChLC) cell for phase grating device applications. To utilize stable ULH state, we set the pitches ChLCs as half gap to obtain fingerprint texture with homeotropic anchoring. With given period, diffraction efficiency can be maximized by optimizing gap. found that fabricated provide large angle 10°...

XAO peptide (Ac-X2A7O2-NH2; X: diaminobutyric acid side chain, -CH2CH2NH3(+); O: ornithine side chain, -CH2CH2CH2NH3(+)) in aqueous solution shows a predominantly polyproline II (PPII) conformation without any detectable α-helix-like conformations. Here we demonstrate by using circular dichroism (CD), ultraviolet resonance Raman (UVRR) and nuclear magnetic resonance (NMR) spectroscopy that sodi...

The one-dimensional problem of selecting the triple helix with the highest volume fraction is solved and hence the condition for a helix to be close-packed is obtained. The close-packed triple helix is shown to have a pitch angle of vCP = 43.3 ◦. Contrary to the conventional notion, we suggest that close packing form the underlying principle behind the structure of collagen, and the implication...

Studying gear power loss theoretically and determining the efficiency of a helical drive depend on many geometrical working parameters, such as rotation speed, tooth number, ratio, helix angle, torque, among others. In this paper, Plackett–Burman screening design Box–Behnken response-surface method are used to consider how above parameters influence efficiency, experimental models provided eval...

Here, we report the three-dimensional structure of severe acute respiratory syndrome coronavirus (SARS-CoV) nsP7, a component of the SARS-CoV replicase polyprotein. The coronavirus replicase carries out regulatory tasks involved in the maintenance, transcription, and replication of the coronavirus genome. nsP7 was found to assume a compact architecture in solution, which is comprised primarily ...

Alpha4 is a regulatory subunit of the protein phosphatase family of enzymes and plays an essential role in regulating the catalytic subunit of PP2A (PP2Ac) within the rapamycin-sensitive signaling pathway. Alpha4 also interacts with MID1, a microtubule-associated ubiquitin E3 ligase that appears to regulate the function of PP2A. The C-terminal region of alpha4 plays a key role in the binding in...

To probe the potential for activity in unevolved amino acid sequence space, we created a third generation combinatorial library of de novo four-helix bundle proteins. The "artificial superfamily" of helical bundles was designed using binary patterning of polar and nonpolar residues, and expressed in Escherichia coli from a library of synthetic genes. WA20, picked from the library, is one of the...