A combined approach, comprising PCR screening and genome mining, was used to unravel the diversity and phylogeny of genes encoding 5-aminolevulinic acid synthases (ALASs, hemA gene products) in streptomycetes-related strains. In actinomycetes, these genes were believed to be directly connected with the production of secondary metabolites carrying the C5N unit, 2-amino-3-hydroxycyclopent-2-enone...

We designed three experimental 10-methacryloyloxydecyl dihydrogen phosphate (MDP)-based one-step (EX) adhesives consisting of MDP, urethane dimethacrylate, and triethylene glycol dimethacrylate adhesives with different water contents (98.4, 196.8, and 294.4 mg/g), and 4-methacryloyloxyethyl trimellitic anhydride (4-META) or 2-hydroxyethyl methacrylate (HEMA)-containing onestep adhesive. The eff...

The usage of amorphous solids in practical applications, such as in medication, is commonly limited by the poor long-term stability of this state, because unwanted crystalline transitions occur. In this study, three different polymeric coatings are investigated for their ability to stabilize amorphous films of the model drug clotrimazole and to protect against thermally induced transitions. For...

As resin-based composites (RBC) replace dental amalgam for environmental reasons, there is a requirement to understand the impact of this alternative restorative material. In study we standardize simultaneous detection five monomeric components associated with RBCs using high performance liquid chromatography (HPLC) coupled solid-phase microextraction (SPME). Factors affecting method (detection...

New drug delivery systems for wound healing applications based on α/β/γ-cyclodextrin (α/β/γ-CD) acrylic (A) and styrenic (S) monomers have been synthesized co-polymerized with 2-hydroxyethyl methacrylate (HEMA) via a cryo-polymerization technique.

We explored the potentials of microarray printing using quill-like microcantilevers onto solid supports that are typically used in microspot printing, including paper, polymeric nitrocellulose and nylon membranes. We compared these membranes with a novel porous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) support (HEMA) with narrow pore size distribution in the 150 nm range, whi...

BACKGROUND Corynebacterium glutamicum is generally regarded as a safe microorganism and is used to produce many biochemicals, including L-glutamate. 5-Aminolevulinic acid (ALA) is an L-glutamate derived non-protein amino acid, and is widely applied in fields such as medicine and agriculture. RESULTS The products of the gltX, hemA, and hemL genes participate in the synthesis of ALA from L-glut...

The first committed step in the biosynthesis of heme, an important cofactor of two catalases and a number of cytochromes, is catalyzed by the hemA gene product. Salmonella enterica serovar Typhimurium hemA26::Tn10d (hemA26) was identified in a genetic screen of insertion mutants that were sensitive to hydrogen peroxide. Here we show that the hemA26 mutant respires at half the rate of wild-type ...

AIM This randomized, double blind, split mouth study was aimed to compare three dentin desensitizing treatment modalities. METHODS Two hundred sixty teeth of 25 patients; each having at least 2 hypersensitive teeth in each quadrant, were included. Teeth were randomized to 4 groups: Group A treated with 2% NaF solution, Group B received GLUMA®; an aqueous solution of Hydroxy-Ethyl-Methacrylate...

The primary aim of this article was to improve the solubility and bioavailability drugs nifedipine, niclosamide, furosemide due their poor dissolution rate. Therefore, these require improvement in rate formulation development, especially solid dosage forms such as hydrogels capsules. Hydrogel structures were synthesized by using a biocompatible, nontoxic, protective pure 2-hydroxy ethyl methacr...