Initial rate studies were carried out using solubilized bovine brain hexokinase. Both ATP 4and 0i, y-5'-adenylyl methylene diphosphonate are competitive inhibitors of MgATP 2and mixed inhibitors of the sugar substrate, while N-acetylglucosamine, a competitive inhibitor of fructose, is a mixed inhibitor of MgATP 2. These results are consistent with a random Bi Bi mechanism for the brain enzyme. ...

The mutant Penicillium chrysogenum strain dogR5, derived from strain AS-P-78, does not respond to glucose regulation of penicillin biosynthesis and β-galactosidase, and is partially deficient in D-glucose phosphorilating activity. We have transformed strain dogR5 with the (hexokinase) hxk2 gene from Saccharomyces cerevisiae. Transformants recovered glucose control of penicillin biosynthesis in ...

The enzymes responsible for the phosphorylation of hexoses in the plant cytosolic fraction of soybean (Glycine max L. Merr cv Williams) nodules have been studied and a hexokinase (ATP:d-hexose 6-phosphotransferase EC 2.7.1.1) and fructokinase (ATP:d-fructose 6-phosphotransferase EC 2.7.1.4) shown to be involved. The plant cytosolic hexokinase had optimum activity from pH 8.2 to 8.9 and the enzy...

Rogosa, M., (National Institutes of Health, Bethesda, Md.), M. I. Krichevsky, and F. S. Bishop. Truncated glycolytic system in Veillonella. J. Bacteriol. 90:164-171. 1965.-Intact Veillonella cells do not utilize carbohydrates for growth, nor are carbohydrates fermented. In cell extracts, there is no detectable glucokinase or fructokinase. Cell extracts do not degrade glucose or fructose unless ...

the ketokinases from different sources differ so widely (liver <0-5 mm and rabbit muscle 0-2M; Hers, 1955). The constant calculated for the locustmuscle enzyme, 5-5 mm, is of the same order as that found for hexokinase from yeast, 1-7 mm, by Slein et al. (1950). We consider that locust muscle contains a single, non-specific hexokinase and that the enzyme is similar to that found in yeast and in...

It is well established that cancer cells differ from most somatic adult cells by their high rate of aerobic glycolysis despite oxidative phosphorylation. This distinct property of cancer cells is often used for tumor imaging in vivo following the administration of the labeled glucose analog, fluoro deoxy glucose (FDG). The high uptake of the labeled FDG in cancer cells can then be detected by p...

A type C hexokinase (ATP:D-hexose-6-phosphotransferase EC 2.7.1.1) was partially purified from the liver of the frog Calyptocephalella caudiverbera. The enzyme is inhibited by glucose levels in the range of normal blood sugar concentrations. The extent of the inhibition by glucose depends on the concentration of ATP, being most marked between 1 and 5 mM ATP. Fructose, although a substrate, was ...

The metabolism of mannose by human erythrocytes has been investigated. Phosphorylation of mannose is achieved by an enzyme with electrophoretic mobility on starch gel indistinguishable from the glucose-phosphorylating enzyme. Mannose phosphorylation is competitively inhibited by glucose; glucose phosphorylation is competitively inhibited by mannose. The K(i) values of inhibition are similar to ...