Our study aimed to compare insights on the dynamics and functional meaning of remodeling 3D higher-order chromatin structure from studies using microscopy analysis FISH experiments genomic utilizing high-throughput chromosome conformation capture assays. We have performed publicly available data several developmentally controlled loci harboring genes important for keratinocyte differentiation. ...

Genome-wide chromosome conformation capture techniques such as Hi-C enable the generation of 3D genome contact maps and offer new pathways toward understanding the spatial organization of genome. One specific feature of the 3D organization is known as topologically associating domains (TADs), which are densely interacting, contiguous chromatin regions playing important roles in regulating gene ...

ST0753, the orthologous gene of Type 1 RNase H found in a thermoacidophilic archaeon, Sulfolobus tokodaii, was analyzed. The recombinant ST0753 protein exhibited RNase H activity in both in vivo and in vitro assays. The protein expressed in an RNase H-deficient mutant Escherichia coli strain functioned to suppress the temperature-sensitive phenotype associated with the lack of RNase H. The in v...

The term Hi-Tech Hi Touch was coined by John Naisbitt in the early 80’s. Since then his concept about the conscious integration of technology into our lives became even more relevant. After the general approach of Hi-Tech Hi-Touch is presented, the rational for applying it specifically to wearable computing is discussed. It is suggested that careful consideration of the human, social and organi...

The process of gene assembly in ciliates, an ancient group of organisms, is one of the most complex instances of DNA manipulation known in any organism. Three molecular operations (ld, hi, and dlad) have been postulated for the gene assembly process, [3], [1]. We propose in this paper a mathematical model for contextual variants of ld and dlad on strings: recombinations can be done only if cert...

Gene flow between genetically distinct populations creates linkage disequilibrium (admixture linkage disequilibrium [ALD]) among all loci (linked and unlinked) that have different allele frequencies in the founding populations. We have explored the distribution of ALD by using computer simulation of two extreme models of admixture: the hybrid-isolation (HI) model, in which admixture occurs in a...

Heterotheca inuloides Cass. (Asteraceae) is used in the traditional medicine of Mexico. The aqueous extract obtained from the flowers of H. inuloides was assessed for anti-inflammatory activity by carrageenan-induced edema test. At 100 mg/kg, i.p, it produced 29% inhibition of inflammation. Ethyl ether (HI-1), butanol (HI-2) and aqueous fraction (HI-3) were obtained from the aqueous extract. Th...

The intramolecular gene assembly model, [1], uses three molecular recombination operations ld, dlad, and hi. A computing model with two contextual recombination operations del and trl , which are based on ld and dlad, respectively, is considered in [6] and its computational power is investigated. In the present paper, we expand the computing model with a new molecular operation such as cpy copy...