نتایج جستجو برای: high resolution melting
تعداد نتایج: 2204548 فیلتر نتایج به سال:
BACKGROUND DNA melting analysis for genotyping and mutation scanning of PCR products by use of high-resolution instruments with special "saturation" dyes has recently been reported. The comparative performance of other instruments and dyes has not been evaluated. METHODS A 110-bp fragment of the beta-globin gene including the sickle cell anemia locus (A17T) was amplified by PCR in the presenc...
Featured Article: Wittwer CT, Reed GH, Gundry CN, Vandersteen JG, Pryor RJ. High-resolution genotyping by amplicon melting analysis using LCGreen. Clin Chem 2003;49:853– 60. DNA melting (the denaturation of DNA by heat) is a fundamental property of DNA that can be used as an analytical tool. Classic studies of DNA melting monitored the absorbance near 260 nm as the temperature was increased and...
High resolution melting profiles of four linearised plasmids (pUC9, pGV403, pHP2, and pBR322) were recorded by means of UV absorbance vs. temperature scanning. The set of transitions obtained for each plasmid are compared to each other and to the transitions obtained for their excised particular antibiotica resistance gene. It can be shown that each gene leaves a characteristic mark on the melt...
The melting transition of finite three-dimensional dust clouds (Yukawa balls) from a solid-like to a liquid-like state is systematically studied with high spatial and temporal resolution of the individual grains by means of stereoscopy. Two different melting scenarios are reported: Melting is induced first by an increase of plasma power, and second by laser-induced heating. The experiments conf...
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