In theory, plasmids can only be maintained in a population when the rate of horizontal gene transfer is larger than the combined effect of segregational loss and the decrease of fitness associated with plasmid carriage. Recent advances in genome sequencing have shown, however, that a large fraction of plasmids do not carry the genes necessary for conjugation or mobilization. So, how are so-call...

the aim of this paper is to study the high order difference scheme for the solution of a fractional partial differential equation (pde) in the electroanalytical chemistry. the space fractional derivative is described in the riemann-liouville sense. in the proposed scheme we discretize the space derivative with a fourth-order compact scheme and use the grunwald- letnikov discretization of the ri...

OBJECTIVES A plasmid-encoded multidrug efflux pump, OqxAB, identified in Escherichia coli of porcine origin, was tested for substrate specificity against selected antibiotics, detergents and disinfectants. The ability of horizontal transfer to food-borne pathogens of the Enterobacteriaceae family was also investigated. METHODS The MICs of selected substrates were determined with a broth dilut...

We describe here a dual-labeling technique involving the green fluorescent protein (GFP) and the red fluorescent protein (DsRed) for in situ monitoring of horizontal gene transfer via conjugation. A GFPmut3b-tagged derivative of narrow-host-range TOL plasmid (pWWO) was delivered to Pseudomonas putida KT2442, which was chromosomally labeled with dsRed by transposon insertion via biparental matin...

in this article, a numerical approximation to the solution of the newell-whitehead equation (nwe) and cauchy problem of ill-posed non-linear diffusion equation have been studied. the presented scheme is obtained by using the derivative of the cubic b-spline quasi-interpolation (bsqi) to approximate the spatial derivative of the dependent variable and first order forward difference to approximat...

[1] A new three-dimensional thermomechanically coupled ice sheet model is developed. Contrary to the majority of three-dimensional ice sheet models (shallow ice approximation), higher-order stress gradients (longitudinal and transverse) are accounted for in the force balance equations. The horizontal velocity field is determined from the force balance equations in their ‘‘derivative form’’ (ell...

Casing coupling location signals provided by the magnetic localizer in retractors are typically used to ascertain the position of casing couplings in horizontal wells. However, the casing coupling location signal is usually submerged in noise, which will result in the failure of casing coupling detection under the harsh logging environment conditions. The limitation of Shannon wavelet time entr...

This paper presents a new method for edge detection using one-dimensional processing. The Discrete Hilbert Transform of a Gaussian function is used as an edge detection lter. The image is smoothed using 1-D Gaussian along the horizontal (or vertical) scan lines to reduce noise. Detection lter is then used in the orthogonal direction, i.e., along vertical (or horizontal) scan lines to detect the...

The non-hydrostatic (NH) compressible Euler equations of dry atmosphere are solved in a simplified two dimensional (2-D) slice framework employing a spectral element method (SEM) for the horizontal discretization and a finite difference method (FDM) for the vertical discretization. The SEM uses high-order nodal basis functions asso-5 ciated with Lagrange polynomials based on Gauss–Lobatto–Legen...