Osmanthus fragrans Lour. has traditionally been a popular ornamental plant in China. In this study, ethanol extracts of the leaves of four cultivar groups of O. fragrans were analyzed by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) and high-performance liquid chromatography with electrospray ionization and mass spectrometry (HPLC-ESI-MS). The results sugg...

An HPLC–DAD–ESI–MSn method has been developed for simultaneous quantification of eight major compounds in eight Saussurea species which have long been used as the traditional Tibetan medicines. The method was validated for sensitivity, precision, and accuracy. LODs were from 0.11 to 5.01 μg mL−1, overall intra-day and inter-day variation was less than 2.70%, and overall recovery was over 98.0%....

Purpose: To develop, validate and compare two chromatographic methods - high performance liquid chromatography with diode array detector ((HPLC-DAD) ultraviolet detection (UPLC-UV) for the effective analysis of polyphenols in Moringa oleifera leaves.Methods: HPLC-DAD UPLC-UV were applied accurate determination eleven major leaves. The conditions was determined on C18 column by gradient elution ...

Abstract Four antibiotics, oxytetracycline, tetracycline, doxycycline, and chlortetracycline were separated quantified in medicated feed. Tetracyclines from feed samples extracted with 0.01 M citric buffer acetonitrile (pH 3.0) further purified 0.45 µm syringe filters. The extract was on commercial RP-C18 column analyzing using liquid chromatograph (LC) two different detectors: diode array dete...

In the present study, various mixtures of aqueous ethanol and acetone solutions were utilized to extract anthocyanins from eggplant peel by a discontinuous process to obtain a natural red-purple colorant. First, the most appropriate combination for extraction solvent was selected as ethanol 70 wt in water acidified with acetic acid 3 wt, which was determined by analysis of extraction yields and...

In Europe, the cultivation of blood oranges for fresh consumption is increasing due to their excellent organoleptic and nutraceutical properties, which give this fruit status functional food. Blood orange has a higher content bioactive compounds, confer additional benefits human health. The main morphological qualitative parameters were studied together with primary metabolites by 1H NMR second...

This study aims to characterise the seeds of two varieties (Beldia and Critical) hemp grown in four geographical areas northern Morocco regarding their phenolic compounds antioxidant power. Thirty-three were identified using HPLC-DAD/ESI-MS2 technique. The profiles all from regions characterised by richness N-trans-caffeoyltyramine (390.22–721.41 ?g g?1), cannabisin A (217.96–393.37 CTE g?1) B ...