A sensitive, simple, and accurate method for the determination and pharmacokinetic study of vanillic acid in rat plasma was developed using reverse-phase HPLC with UV detection after oral administration of the traditional Chinese medicine preparation of the Di-Gu-Pi decoction. Plasma samples taken from rats were extracted with methanol. The constituent vanillic acid was separated on a C(18) sta...

Analytical methods to check the purity of active pharmaceuticals have been described in pharmacopeias. HPLC-UV detection is the most common analytical liquid chromatography technique for impurity profiling in QC laboratories because of its ease-of-use, robustness, and reliability. However, there are many occasions where ultraviolet (UV) detection has limitations, including cases where analytes ...

An HPLC-UV method was developed and validated for the determination of lumefantrine in human plasma. Lumefantrine and its internal standard halofantrine were extracted from plasma samples using protein precipitation with acetonitrile (0.2% perchloric acid) followed by solid-phase extraction with Hypersep C(8) cartridges. Chromatographic separation was performed on a Zorbax SB-CN HPLC column (3....

Instrumental bitterness assessment of traditional Chinese herbal medicine (TCM) preparations was addressed in this study. Three different approaches were evaluated, high-performance liquid chromatography coupled to UV detector (HPLC), capillary electrophoresis coupled to UV detector (CE) and a potentiometric multisensor system - electronic tongue (ET). Most studies involving HPLC and CE separat...

A cloud point extraction coupled with high performance liquid chromatography (HPLC/UV) method was developed for the determination of Δ(9)-tetrahydrocannabinol (THC) in micellar phase. The nonionic surfactant "Dowfax 20B102" was used to extract and pre-concentrate THC from cannabis resin, prior to its determination with a HPLC-UV system (diode array detector) with isocratic elution. The paramete...

The aim of current investigation was the validation of HPLC method with UV-detection for identification and determination of Yohimbine by using column Spherisorb ODS RP C18 (250 mm x 4.6 mm x 5 μm), temperature: 25 oC, mobile phase: methanol : acetonitrile : water = 70 : 20 : 10 v/v, flow rate: 2 ml/min., UV-detection: λ = 270 nm. HPLC method was validated in accordance of linearity, limit of d...

ABSTRACT Artesunate (ART) is a readily available anti malarial in combination therapy. The assay method has posed a challenge because it does not have a readily recognisable absorption chromophore needed for UV spectroscopy. A simple and rapid assay method involving two reaction steps has been developed for assay of ART in pharmaceutical formulations. The method involves basic reaction of ethan...

The power of traditional high-performance liquid chromatography with UV detection (HPLC-UV, DAD) can be readily extended by simply coupling a mass spectrometer (MS) to an existing system. In the early years of liquid chromatographic mass spectrometry (LC-MS), this coupling was considered exotic and complex. After more than 15 years of refinement, LC-MS systems are robust and easy to use, and pr...

This method was developed for the determination of melamine in milk powder and egg. Solid phase extraction (SPE) and HPLC/UV are used consistent with the Chinese regulatory method. The sample preparation is performed using a polymeric mixed mode strong cation exchange resin. The separation and detection are performed by HPLC/UV. The limit of detection is 10 μg/kg. Linear calibration curves were...