objective(s):hypoxia induces cellular oxidative stress that is associated with neurodegenerative diseases. here, the protective effects of ferulic acid (fa) on hypoxia-induced neurotoxicity in pc12 cells were evaluated. materials and methods:we investigated the effect of fa on pc12 cells subjected to hypoxia stress, in vitro. results:fa increased cell viability, prevented membrane damage (ldh r...

Background: Apoptosis is a process that plays an important role during early stage of implantation. The aim of this study was to investigate the incidence of apoptosis in mice endometrium after ovarian stimulation at implantation period. Methods: NMRI female mice were divided into two groups: 1) control group, which were rendered pseudopregnant by vaginal stimulation and 2) experimental group, ...

apoptosis has a critical role in the pathogenesis of bleomycin induced-pulmonary fibrosis. the severity of fibrosis varies among different strains of mice. recent studies have indicated that expression of apoptotic regulatory genes may be specific in different cell types in various strains. in this study, bleomycin-induced pulmonary apoptosis in nmri albino mice were compared with c57bl/6 black...

We have shown previously that the death receptor CD95 could contribute to anticancer drug-induced apoptosis of colon cancer cells. In addition, anticancer drugs cooperate with CD95 cognate ligand or agonistic antibodies to trigger cancer cell apoptosis. In the present study, we show that the anticancer drug cisplatin induces clustering of CD95 at the surface of the human colon cancer cell line ...

BACKGROUND Modes of cell death have been defined morphologically as apoptosis and oncosis. Infarcted myocytes have been reported to show apoptosis, as revealed by DNA fragmentation by DNA ladder and by in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) at the light microscopic level. We investigated whether TUNEL-positive infarcted myocytes have apoptotic or o...

The use of nanoparticles (NPs) has been extended to many fields such as agriculture, food industry, medicine and biotechnological fields. Thereby, human exposure to NPs consequently increases. Therefore, there is a concern about the potential biological effects and toxicity of NPs for humans and the environment. This study aimed to investigate the cytotoxicity effects of magnesium oxide (MgO) a...

To investigate the in vitro inhibitory effect of rAd-ODC/Ex3as on human esophageal carcinoma cells. The infection rate of rAd-ODC/Ex3as was measured with the aid of GFP expression. Western Blot technique was used to observe the inhibition of ODC expression in infected tumor cells. The malignant phenotype of Eca109 cell line was assessed by growth curve. TUNEL was used to analyze cell apoptosis....

Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of olives. We have previously reported that MA induces apoptotic cell death via the mitochondrial apoptotic pathway in HT29 colon cancer cells. Here, we show that MA induces apoptosis in Caco-2 colon cancer cells via the extrinsic apoptotic pathway in a dose-dependent manner. MA triggered a series of effe...

We investigated the effects of 2'-deoxyinosine (d-Ino), a modulator yielding thymidine phosphorylase activity, on cellular pharmacology of 5-fluorouracil (FUra) in various human colorectal cell lines and its antitumoral activity when combined with FUra in human xenografts. Associating d-Ino with FUra increased by 38 up to 700 times the sensitivity of HT29 and FUra-resistant SW620 lines, respect...

One hallmark of apoptosis is the degradation of chromosomal DNA. We cloned the Caenorhabditis elegans gene nuc-1, which is involved in the degradation of the DNA of apoptotic cells, and found that nuc-1 encodes a homolog of mammalian DNase II. We used the TUNEL technique to assay DNA degradation in nuc-1 and other mutants defective in programmed cell death and discovered that TUNEL labels apopt...