OBJECTIVE To describe the natural history, antenatal and postnatal therapy, and clinical outcomes of Australian patients with fetomaternal/neonatal alloimmune thrombocytopenia (NAIT) recorded in the Australian NAIT registry. METHODS Analysis of registry data of Australian mothers treated antenatally for NAIT and any fetus/newborn with thrombocytopenia (TCP) and maternal human platelet antigen...

Human platelet membranes were prepared by the glycerol-lysis technique of Barber and Jamieson (Barber, A. J., and Jamieson, G. A. (1970) J. Biol. Chem 245, 6357-6365), solubilized in 1% Triton X-100 and subjected to crossed immunoelectrophoresis, in 1% agarose, employing a rabbit anti-human platelet membrane antibody in the second dimension. Approximately 20 immunoprecipitates (numbered 1 to ...

OBJECTIVES To determine the frequency of alloimmunization against human platelet antigens (HPAs) and human leucocyte antigen class 1 (HLA1) in multiparous women and multi-transfused patients.   METHODS This prospective study was conducted between January and August 2013, on 50 multiparous women with no history of previous blood transfusion recruited from the Obstetrics and Gynecology Clinic, ...

BACKGROUND AND PURPOSE The role of polymorphisms of the platelet glycoprotein (GP) IIb/IIIa receptor in the development of cardiovascular disease has been the subject of intensive research. The aim of this study was to determine the association of the HPA-3 polymorphism of platelet GPIIb with ischemic stroke and subsequent survival and to identify possible interactions of HPA-3 with classic ris...

Background Studies with mouse models demonstrate 2 pathways of systemic anaphylaxis: a classic pathway mediated by IgE, FcεRI, mast cells, histamine, and platelet-activating factor (PAF) and an alternative pathway mediated by total IgG, FcgRIII, macrophages, and PAF. The former requiring fewer antigens and antibodies than the latter. The importance of the alternative pathway in humans is uncert...

Platelets express a variety of immunogenic markers on the cell surface. Some of these antigens are shared with other cell types as in the case of HLA antigens, which are shared with virtually all nucleated cells in the body, whereas others are observed to be essentially platelet specific. This chapter reviews the antigens expressed on platelets, the various patterns of alloimmunization to these...