Anaerobic bacteria were isolated from industrial wastewater and soil samples and tested for exoelectrogenic activity by current production in double chambered microbial fuel cell (MFC), which was further transitioned into a single chambered microbial electrolytic cell to test hydrogen production by electrohydrogenesis. Of all the cultures, the isolate from industrial water sample showed the max...

Biological conversion of solar energy into hydrogen is naturally realized by some microalgae species due to a coupling between the photosynthetic electron transport chain and a plastidial hydrogenase. While promising for the production of clean and sustainable hydrogen, this process requires improvement to be economically viable. Two pathways, called direct and indirect photoproduction, lead to...

In this study, the effects of hydrogen and hydroxygen additions and oxygen enrichment on the emission characteristics of a gasoline engine (EF7) were investigated and compared with each other. The simulation was launched by GT-Power at different engine speeds with 5 % to 15 % volume fractions for both of oxygen and hydrogen enrichment and 4.5 % to 9 % volume fractions of hydroxygen addition in ...

14 This study investigates the mesophilic biohydrogen production from glucose using a strictly 15 anaerobic strain, Clostridium butyricum CWBI1009, immobilized in a trickling bed sequenced 16 batch reactor (TBSBR) packed with a Lantec HD Q-PAC ® packing material (132 ft2/ft3 specific 17 surface). The reactor was operated for 62 days. The main parameters measured here were 18 hydrogen compositio...

Escherichia coli can perform at least two modes of anaerobic hydrogen metabolism and expresses at least two types of hydrogenase activity. Respiratory hydrogen oxidation is catalysed by two ‘uptake’ hydrogenase isoenzymes, hydrogenases -1 and -2, and fermentative hydrogen production is catalysed by hydrogenase-3. Harnessing and enhancing the metabolic capability of Escherichia coli to perform a...

Quantitative detection of hydrogen peroxide in solution above a Streptococcus gordonii (Sg) bacterial biofilm was studied in real time by scanning electrochemical microscopy (SECM). The concentration of hydrogen peroxide was determined to be 0.7 mM to 1.6 mM in the presence of 10 mM glucose over a period of 2 to 8 h. The hydrogen peroxide production measured was higher near the biofilm surface ...

Escherichia coli can perform at least two modes of anaerobic hydrogen metabolism and expresses at least two types of hydrogenase activity. Respiratory hydrogen oxidation is catalysed by two 'uptake' hydrogenase isoenzymes, hydrogenase -1 and -2 (Hyd-1 and -2), and fermentative hydrogen production is catalysed by Hyd-3. Harnessing and enhancing the metabolic capability of E. coli to perform anae...

Microbial mats containing the filamentous anoxygenic photosynthetic bacterium Chloroflexus aggregans develop at Nakabusa hot spring in Japan. Under anaerobic conditions in these mats, interspecies interaction between sulfate-reducing bacteria as sulfide producers and C. aggregans as a sulfide consumer has been proposed to constitute a sulfur cycle; however, the electron donor utilized for micro...

This paper summarizes R&D activities and progress at NORAM Engineering and the University of British Columbia (UBC) on preparation and testing of thin palladium-based membranes and their applications. Most of these activities were carried out internally at NORAM, some jointly with UBC and their spin-off company, Membrane Reactor Technology (MRT) through a wide range of projects. Key results out...

Hydrogen is a clean and efficient fuel, considered as a potential and more sustainable energy substitute for fossil fuels. Biological hydrogen production stands out as an eco friendly process carried out under mild operating conditions with renewable resources. In the current work laboratory scale production of hydrogen using phototrophic purple non-sulphur bacteria Rhodobacter sphaeroides, ana...