The method has been developed and validated for the determination of hydroquinone in liposomal formulation. The samples were dissolved in methanol and evaluated in 293 nm. The validation parameters such as linearity, accuracy, precision, specificity, limit of detection (LOD) and limit of quantitation (LOQ) were determined. The calibration curve was linear in 1-50 µg /mL range of hydroquinone an...

The conclusions stated after evaluation of 49 test results are presented in Table 1 for the in vivo mutagenicity and in Table 2 for in vitro mutagenicity. To evaluate the mutagenicity of the product, the criteria in point 4.2.2 of the Annex VI to the Directive 67/548/EEC are used. The tests using intra-peritoneal administration are not taken into account to determine the mutagenicity of hydroqu...

Benzene toxicity is attributed to its metabolism, which is primarily mediated by the ethanol-inducible cytochrome P450 2E1 isoform (CYP2E1). The present study investigated the myelotoxicity and urinary concentrations of major benzene metabolites in adult CD-1 male mice treated with low levels of benzene vapors, ethanol, or a combination of the two. Groups of ethanol-treated (5% in a Lieber-DeCa...

We previously reported evidence that humans metabolize benzene via two enzymes, including a hitherto unrecognized high-affinity enzyme that was responsible for an estimated 73% of total urinary metabolites [sum of phenol (PH), hydroquinone (HQ), catechol (CA), E,E-muconic acid (MA), and S-phenylmercapturic acid (SPMA)] in nonsmoking females exposed to benzene at sub-saturating (ppb) air concent...

The great opportunities represented by exosomes in liquid biopsy diagnostics and the relevance of CD147 protein as diagnostic prognostic cancer biomarker led us to develop first bio-electroanalytical platform for determination exosomal (exoCD147) exploiting micro-sized magnetic beads coated with specific anti-CD147 antibodies. captured target was sandwiched biotin functionalized detector antibo...

one of the most important parameters describing the liposomal formulation of hydroquinone is encapsulation efficacy. for the efficacy evaluation of hydroquinone trapped in liposomal structure, there is a need to first separate liposome from the matrix surrounding it. there are various separation techniques; however, in this study, the three techniques of centrifuges with and without washing and...

The method has been developed and validated for the determination of hydroquinone in liposomal formulation. The samples were dissolved in methanol and evaluated in 293 nm. The validation parameters such as linearity, accuracy, precision, specificity, limit of detection (LOD) and limit of quantitation (LOQ) were determined. The calibration curve was linear in 1-50 µg /mL range of hydroquinone an...

SYNOPSIS INTRODUCTION Brain-based learning, a recent approach in the educational literature, is related with the structure and the relation of the functioning of the brain with learning. The pioneers of this approach (Caine & Caine, 1994) have come up with principles about brain and learning. Another recent approach, constructivism, is also concerned with teaching and learning and has brought i...

Solid tumors have been reported in the Zymbal gland, oral and nasal cavities, liver, and mammary gland of Sprague-Dawley rats following chronic, high-dose administration of benzene. The carcinogenic activity of benzene is thought to be caused by activation to toxic metabolites that can interact with DNA, forming covalent adducts. A nuclease P1-enhanced 32P-postlabeling assay, having a sensitivi...