The bioassay-directed isolation of a marine brown alga, Ecklonia cava, afforded four phlorotannin derivatives, eckol (1), 8,8'-bieckol (2), 8,4"'-dieckol (3), and phlorofucofuroeckol A (4). Among these compounds, 2 and 3 exhibited an inhibitory effect on human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) and protease. Specifically, they inhibited the RT more potently than th...

Background: Group A Streptococcus (GAS) causes a wide array of clinical manifestations ranging from mild pharyngitis to suppurative and non-suppurative severe debilitating diseases. Hence, simple, rapid detection method with high sensitivity specificity is needed. Objectives: This study embarked on the visual streptococcal pyrogenic exotoxin B (speB) gene by real-time turbidimetry loop-mediated...

As a matter of fact, infectious diseases hamper everyone’s life and produce lifelong threat to everyone neutral age, sex, lifestyle socio-economic status. Nowadays, come into the sight Chikungunya viral (CHIKV) infection injured many Asian African countries, also deliberated in rising countries low countries. CHIKV is positive-sense, enveloped single-stranded, RNA virus belonging genus Alphavir...

The catechin, epigallocatechin gallate (eGCG), found in green tea, has inhibitory activity against a number of protein toxins and was investigated in relation to its impact upon ricin toxin (RT) in vitro. The IC(50) for RT was 0.08±0.004 ng/mL whereas the IC(50) for RT+100 μM eGCG was 3.02±0.572 ng/mL, indicating that eGCG mediated a significant (p<0.0001) reduction in ricin toxicity. This expe...

Diagnostic systems based on reverse transcription (RT)-PCR are widely used for the detection of viral genomes in different human specimens. The application of internal controls (IC) to monitor each step of nucleic acid amplification is necessary to prevent false-negative results due to inhibition or human error. In this study, we designed various real-time RT-PCRs utilizing the coliphage MS2 re...

We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcriptio...

Objectives Physical activity is associated with improvement in overall health and well-being, but robust evidence comprehensive assessment of general lacking. This study aimed to clarify the effects physical on intrinsic capacity among community-dwelling older adults subjective memory concerns. Design A single-blind randomized controlled trial compared aerobic training (AT), resistance (RT), co...

An artificial intelligence-assisted low-cost portable device for the rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is presented here. This standalone temperature-controlled houses tubes designed conducting reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays. Moreover, utilises illuminated by LEDs, an in-built camera, and a small onboar...

Nucleic acid amplification is the basis for many molecular diagnostic assays. In these cases, the amplification product must be detected and analyzed, typically requiring extended workflow time, sophisticated equipment, or both. Here we present a novel method of amplification detection that harnesses the pH change resulting from amplification reactions performed with minimal buffering capacity....