We modified the Karnovsky and Roots method of staining sites of acetylcholinesterase (AChE) activity at neuromuscular junctions (NMJs) to survive the lengthy, multiple steps of in situ hybridization and autoradiography. When the original method of Karnovsky and Roots is used to identify the muscle endplates, the stain does not survive the in situ hybridization procedures and association of mRNA...

Given input-output pairs of an elliptic partial differential equation (PDE) in three dimensions, we derive the first theoretically-rigorous scheme for learning associated Green's function $G$. By exploiting hierarchical low-rank structure $G$, show that one can construct approximant to $G$ converges almost surely and achieves a relative error $\mathcal{O}(\Gamma_\epsilon^{-1/2}\log^3(1/\epsilon...

We have mapped the DNase I-hypersensitive sites around the epsilon-globin and c-myc genes in two human leukemia cell lines K562 and HL60. In K562 cells in which the epsilon-globin gene is transcribed, six DNase I-hypersensitive sites are found in 6 kilobases (kb) of upstream flanking DNA; in HL60 cells in which the c-myc gene is expressed, two DNase I-hypersensitive sites are observed in 2 kb o...

Alzheimer disease (AD) is the most frequent cause of dementia in Western countries. Putative genetic risk factors for AD are polymorphisms in the apolipoprotein E (APOE) gene and in the low-density lipoprotein receptor-related protein (LRP) gene. Our objective was to investigate the role of the APOE coding region polymorphisms epsilon 2, epsilon 3, and epsilon 4 and APOE promoter variants A/T a...

Using both analytical solutions obtained from simplified systems and numerical results from more realistic cases, we investigate the role played by the dielectric constant of membrane proteins epsilon(p) and pore water epsilon(w) in permeation of ions across channels. We show that the boundary and its curvature are the crucial factors in determining how an ion's potential energy depends on the ...

We investigate the possibility to describe quark mass hierarchies as well CKM mixing matrix without fine-tuning in a flavour model with modular $A_4$ symmetry. The are considered vicinity of fixed point $\tau = \omega \equiv \exp({i\,2\pi/3})$ (the left cusp fundamental domain group), $\tau$ being VEV modulus. involves forms level 3 and weights 6, 4 2, contains eight constants, only two which, ...

let $l$ be a completely regular frame and $mathcal{r}l$ be the ‎ring of continuous real-valued functions on $l$‎. ‎we show that the‎ ‎lattice $zid(mathcal{r}l)$ of $z$-ideals of $mathcal{r}l$ is a‎ ‎normal coherent yosida frame‎, ‎which extends the corresponding $c(x)$‎ ‎result of mart'{i}nez and zenk‎. ‎this we do by exhibiting‎ ‎$zid(mathcal{r}l)$ as a quotient of $rad(mathcal{r}l)$‎, ‎the‎ ‎...

Human C/EBP epsilon is a newly cloned CCAAT/enhancer-binding transcription factor. Initial studies indicated it may be an important regulator of human myelopoiesis. To elucidate the range of expression of C/EBP epsilon, we used reverse transcription-polymerase chain reaction (RT-PCR) analysis and examined its expression in 28 hematopoietic and 14 nonhematopoietic cell lines, 16 fresh myeloid le...

The high-affinity receptor for immunoglobulin (Ig) E (Fc epsilon RI) on mast cells and basophils plays a key role in IgE-mediated allergies. Fc epsilon RI is composed of one alpha, one beta, and two gamma chains, which are all required for cell surface expression of Fc epsilon RI, but only the alpha chain is involved in the binding to IgE. Fc epsilon RI-IgE interaction is highly species specifi...

In a recent paper, it was shown that, under the action of lateral van der Waals (vdW) force due to perfectly conducting corrugated surface, neutral anisotropic polarizable particle in vacuum can be attracted not only nearest corrugation peak but also valley or an intermediate point between and valley, with such behaviors called peak, regimes, respectively. present we investigate how these regim...