The interaction of RecA protein with short single-stranded oligonucleotides is characterised by flow linear dichroism (LD), isoelectric focusing (IEF) and electron microscopy (EM). From LD and EM it is evident that RecA forms long filaments with at least some 50 oligonucleotides in a 'train formation'. The tendency to form trains is substantially lower when an amino group is attached to the 5' ...

Cytosolic and membrane-associated proteins prepared from rice cells were separated and compared by two different 2D-PAGE methods, isoelectric focusing (IEF)/SDS-PAGE and nonequilibrium pH gradient electrophoresis (NEPHGE)/SDS-PAGE. Although IEF/SDS-PAGE of the cytosolic proteins showed sufficient resolution, some mitochondrial and basic microsomal membrane-associated proteins were weakly or har...

Title of Dissertation: APPLICATION OF REDUCED ORDER MODELING TECHNIQUES TO PROBLEMS IN HEAT CONDUCTION, ISOELECTRIC FOCUSING AND DIFFERENTIAL ALGEBRAIC EQUATIONS Pramod P. Mathai, Doctor of Philosophy, 2008. Dissertation directed by: Professor Benjamin Shapiro Department of Aerospace Engineering This thesis focuses on applying and augmenting ‘Reduced Order Modeling’ (ROM) techniques to large sc...

The article explores the role, essence and significance of international ratings indices, assesses current situation Republic Uzbekistan in context economic reforms, also main directions for improving position indices as a factor stabilizing developing economy introducing foreign experience accordance with changing modern conditions. This discusses ways methods to improve index freedom (IEF). r...

Président du jury : François JULIEN Directeur de Recherches(HDR), IEF – Univ. Paris Sud Rapporteurs : Olivier GAUTHIER-LAFAYE Chargé de Recherches(HDR), LAASUniv. Paul Sabatier Dominique COQUILLAT Directeur de Recherches(HDR), L2C –Univ. Montpellier 2 Examinateurs : Robson FERREIRA Directeur de Recherches(HDR), LPA – ENS de Paris Bloch oscillations and Wannier Stark Ladder study in Semiconducto...

Free flow electrophoresis (FFE) is a fractionation method, based on isoelectric focusing (IEF). We validate the reproducibility of the method and show that it can be applied by biobanks in order to fractionate fluid biospecimens efficiently and reproducibly and to facilitate downstream proteomic applications. We also propose a simple method allowing researchers to assess the reproducibility of ...

This follow-up paper completes the author's investigations to explore the in-solution structural preferences and relative free energies of all OH-substituted oxazole, thiazole, isoxazole, and isothiazole systems. The polarizable continuum dielectric solvent method calculations in the integral-equation formalism (IEF-PCM) were performed at the DFT/B97D/aug-cc-pv(q+(d))z level for the stable neut...