PURPOSE Current immunohistochemical (IHC)-based definitions of luminal A and B breast cancers are imperfect when compared with multigene expression-based assays. In this study, we sought to improve the IHC subtyping by examining the pathologic and gene expression characteristics of genomically defined luminal A and B subtypes. PATIENTS AND METHODS Gene expression and pathologic features were ...

In anatomic pathology, immunohistochemistry (IHC) serves as a diagnostic and prognostic method for identification of disease markers in tissue samples that directly influences classification and grading the disease, influencing patient management. However, till today over most of the world, pathological analysis of tissue samples remained a time-consuming and subjective procedure, wherein the i...

The NCCN Task Force on Estrogen Receptor and Progesterone Receptor Testing in Breast Cancer by Immunohistochemistry was convened to critically evaluate the extent to which the presence of the estrogen receptor (ER) and progesterone receptor (PgR) biomarkers in breast cancer serve as prognostic and predictive factors in the adjuvant and metastatic settings, and the ability of immunohistochemical...

Here, we aimed to identify an immunohistochemical (IHC)-based classifier as a prognostic factor in patients with esophageal squamous cell carcinoma (ESCC). A cohort of 235 patients with ESCC undergoing radical esophagectomy (with complete clinical and pathological information) were enrolled in the study. Using the least absolute shrinkage and selection operator (LASSO) regression model, we extr...

BACKGROUND & OBJECTIVES Mutation of nucleophosmin (NPM1) gene in the absence of FLT3-ITD (FMS related tyrosine kinase 3 - internal tandem duplications) mutation carries a good prognosis in cytogenetically normal acute myeloid leukaemia (AML). NPM1, a multifunctional nucleolar phosphoprotein that shuttles between nucleus and cytoplasm, gets trapped in the cytoplasm when mutated. Immunohistochemi...

AIMS Lynch Syndrome is caused by mutations in DNA mismatch repair (MMR) genes. Mutation carrier identification is facilitated by immunohistochemical detection of the MMR proteins MHL1 and MSH2 in tumour tissue and is desirable as colonoscopic screening reduces mortality. However, protein detection by conventional immunohistochemistry (IHC) is subjective, and quantitative techniques are required...

Introduction: Visual assessment of endomyocardial biopsy (EMB) specimens may result in errors due to the human factor and inability obtain quantitative data on intensity immunohistochemical (IHC) reactions severity pathological changes heart transplant. Detailed digital characterization EMB based automated or semi-automated computer-assisted morphometry improves diagnostic accuracy EMB. Objecti...

The aim of the research was to study possibility using immunohistochemical markers for diagnosis intravital stangulation.
 Materials and methods: Fragments neck skin from strnaglulation zone were selected as objects research. main group included 20 deaths due hanging (10 men 10 women). For control group, cases acute coronary death (5 5 women) used. Using a complex IHC markers, labeling epi...

Background: Distinguishing between pleural epithelioid mesothelioma, lung adenocarcinoma, and poorly differentiated squamous cell carcinoma (SCC) is a challenge in some cases. A panel of markers has been recommended by the International Mesothelioma Interest Group (IMIG) guideline to differentiate mesothelioma from adenocarcinoma SCC. However, use novel highly specific immunohistochemical (IHC)...

Background & Objective: Breast cancer (BC) is the most prevalent disease among women around world, considered world's leading cause of death (15% total deaths) in 2018. β-catenin a multifunctional protein located cytoplasm and/or nucleus cell. Several studies suggested that expression plays critical role invasion and metastasis. This research sought to examine breast its associations with ...