نتایج جستجو برای: in vitro hybridization
تعداد نتایج: 16982643 فیلتر نتایج به سال:
Chromosome malsegregation in peripheral blood lymphocytes of 24 healthy male subjects was analysed by means of fluorescence in situ hybridization with centromeric probes of chromosomes 7, 11, 18 and X. On the basis of the distribution of centromeric signals in cytokinesis-blocked cells, both loss (leading to centromere-positive micronuclei) and non-disjunction (resulting in an unbalanced distri...
The incidence of spontaneous aneuploidy in human somatic and germ cells is known to be positively associated with aging. However, the influence of age on the individual susceptibility to chemically induced chromosome malsegregation has not been elucidated. In this study the spindle poison vinblastine (VBL) was used as a model compound to assess the influence of donor age on chemically induced c...
BACKGROUND Selecting the best embryo for transfer, with the highest chance of achieving a vital pregnancy, is a major goal in current in vitro fertilization (IVF) technology. The high rate of embryonic developmental arrest during IVF treatment is one of the limitations in achieving this goal. Chromosomal abnormalities are possibly linked with chromosomal arrest and selection against abnormal fe...
Testing potential modifiers of the response of tumors to radiation therapy requires large, expensive, and time-consuming clinical trials. It would, therefore, be of value to have a rapid surrogate end point of tumor response that could be used to evaluate such modifiers. We here propose that radiation-induced stable chromosome translocations measured by fluorescence in situ hybridization (FISH)...
With the aim of investigating the potential of flavan-3-ols to influence the growth of intestinal bacterial groups, we have carried out the in vitro fermentation, with human faecal microbiota, of two purified fractions from grape seed extract (GSE): GSE-M (70% monomers and 28% procyanidins) and GSE-O (21% monomers and 78% procyanidins). Samples were collected at 0, 5, 10, 24, 30 and 48 h of fer...
The significance of haploid plants as genetic and plant breeding tools has been recognized for a long time. Haploid production techniques including anther culture, isolated microspore culture and intergeneric hybridization between wheat × Hordeum bulbosum and wheat × maize have been used to produce homozygous lines which accelerate breeding programs. In this study, wheat × maize hybridization a...
Background: The treatment of choice for hydatidosis as an important zoonotic disease is surgery. Different agents are injected into the cyst to prevent secondary hydatidosis. To avoid the side effects of such protoscolicidal agents, considering the high protoscolicidal effects of the garlic extract, we conducted the present study on protoscolices in limited applicable times and compared the ext...
we have analyzed paraffin sections from 55 patients with histologically confirmed hodgkin’s disease (hd) for the presence of epstein-barr virus (ebv) markers using in situ hybridization to detect the ebv-encoded rnas (ebers) and immunohistochemistry to identify latent membrane protein-1 (lmp1) expression. tissue specimens from 55 cases of hodgkin’s disease included 22-mixed cellularity (mc), 27...
background:breast cancer remains the most common and second lethal cancer in females. her-2/neu is one of the most important amplified oncogene in breast cancer. the amplification of her-2 is correlated with decreased survival, metastasis, and early recurrence. the amplification of her-2/neu gene and synthesis of the protein are reported in 10%-34% of breast cancer cases associated with tumor ...
background the incidence rate of gastric cancer in western countries has shown a remarkable decline in the recent years while it is still the most common cancer among males in iran. the proto-oncogene myc, located at 8q24.1, regulates almost 15% of human genes and is activated in 20% of all tumors. the amplification of myc and overexpression of its protein product are observed in 15 - 30% of ga...
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