The influence of test variables on in vitro susceptibility testing of caspofungin was examined with 694 isolates of Candida albicans including seven laboratory-derived glucan synthesis mutants. The conditions providing the greatest separation between the mutant strains and the clinical isolates were RPMI medium, MIC end point criterion of partial inhibition, and incubation for 24 h. These testi...

Antibiotic susceptibility testing of two isolates of the Q-fever agent, Coxiella burnetii, was performed with recently and persistently infected L929 fibroblast cells. The two genetically distinct isolates, Nine Mile and Priscilla, are implicated in two different clinical disease syndromes, acute and chronic Q fever, respectively. We compared the efficacies of rifampin, doxycycline, and five 4-...

Infections with multidrug-resistant Acinetobacter baumannii-Acinetobacter calcoaceticus complex bacteria complicate the care of U.S. military personnel and civilians worldwide. One hundred thirty-three isolates from 89 patients at our facility during 2006 and 2007 were tested by disk diffusion, Etest, and broth microdilution for susceptibility to tetracycline, doxycycline, minocycline, and tige...

Caspofungin (Merck Pharmaceuticals) was tested in vitro against 25 clinical isolates of Coccidoides immitis. In vitro susceptibility testing was performed in accordance with the National Committee for Clinical Laboratory Standards document M38-P guidelines. Two C. immitis isolates for which the caspofungin MICs were different were selected for determination of the minimum effective concentratio...

background: the present study describes the susceptibility of prepubertal testis of mice to prooxidant induced oxidative impairments both under in vitro and in vivo exposure conditions. materials and methods: following in vitro exposure to iron (5,10 and 25 m), oxidative response measured in terms of lipid peroxidation and hydroperoxide levels in testis of pre pubertal mice (4 wk) was more robu...

background: infertility is an increasing medical and social problem. in vitro fertilization (ivf) has become a common and accessible treatment for a wide variety of indications that have variable outcomes. natural killer (nk) cells have been identified as relevant immunological factors involved in reproductive success or failure. objective: the aim of this study was to compare the percentage of...

background: pyridaben, a pyridazinone derivative, is a new acaricide and insecticide for control of mites and some insects such as white flies, aphids and thrips. objective: this study was designed to elucidate how pyridaben can affect the sperms' morphological parameters, its dna integrity, and to estimate the effect of various quantities of pyridaben on in vitro fertilization rate. materials ...

elicitation with middle-viscous chitosan (30 mg/50 ml) significantly stimulated silymarin synthesis in silybum marianum hairy root cultures. the root cultures established by infection with agrobacterium rhizogenes ar15834 showed a potential for production of silymarin. elicitation with medium molecular weight of chitosan (0, 5, 10, 20, and 30 mg/50 ml) was used in order to improve silymarin pro...

background: acanthamoeba keratitis (ak) is potentially a sight-threatening infection and its treatment is challenging. this is mainly due to presence of resistant cyst form. indeed, cysts are highly resistant to current available drugs. chemical drugs are toxic to human keratocytes. it should also be mentioned that most available anti- acanthamoeba drugs are poorly cysticidal, in iran and world...