background: this study was designed to detect whether there is a correlation between in vitro susceptibility of field isolates of leishmania major and the clinical outcomes of meglumine antimoniate (glucantime®) therapy, the main­stay of cutaneous leishmaniasis treatment in iran. methods: forty-three patients infected with l. major were enrolled in this study from october 2009 to march 2010 and...

results the results obtained for each drug were compared with other drugs and also with the results of the control groups. the results related to promastigote and amastigote assays showed that when the dose of both drugs increased, the parasite number is reduced in comparison with the control groups. moreover, the parasitic burden in the test cultures decreased significantly. macrophage assay r...

CRISPR-Cas9 is efficient enough to knock out both alleles directly by introducing out-of-frame mutations. We succeeded in making biallelic on-target frameshift mutations of the endogenous Gli3 gene; however, the GLI3 protein was expressed in all six of the established cell lines carrying homozygous out-of-frame mutations. We developed a dual-tagged expression vector and proved that illegitimate...

objective: to evaluate the efficacy of in vitro sperm activation (isa) using non-liquefied versus liquefied asthenozoospermic semen samples for improvement of sperm parameters. materials and methods: fifty six oligoasthenozoospermic (oa) patients (age range: 22-44 years; mean: 32.089 years) were enrolled in this study. oa patients were classified according to type of infertility. also, duration...

traumatic brain injury (tbi) is caused by rapid deformation of the brain, resulting in a cascade of pathological events and ultimately neurodegeneration. in vitro models of tbi can help us to describe the pathobiological mechanisms. in vitro models of brain injury support a platform for performing repeatable, well-controlled, environmentally isolated experiments. other advantages of in vitro mo...

Bacterial trans translation is activated when translating ribosomes are unable to elongate or terminate properly. Small protein B (SmpB) and transfer-messenger RNA (tmRNA) are the two known factors required for and dedicated to trans translation. tmRNA, encoded by the ssrA gene, is a bifunctional molecule that acts both as a tRNA and as an mRNA during trans translation. The functions of tmRNA e...

The scanning model of RNA translation proposes that highly stable secondary structures within mRNAs can inhibit translation, while structures of lower thermal stability also affect translation if close enough to the 5' methyl G cap. However, only fragmentary information is available about the dependence of translation efficiency in live mammalian cells on the thermodynamic stability, location, ...

Background Male infertility management has made significant progress during the past three decades, especially after the introduction of intracytoplasmic sperm injection (ICSI) in 1992. However, many boys and men still suffer from primary testicular failure due to acquired or genetic causes. New and novel treatments are needed to address these issues. Spermatogenesis originates from spermatogon...