Coronavirus M protein is an essential component of virion and plays pivotal roles in virion assembly, budding and maturation. The M protein is integrated into the viral envelope with three transmembrane domains flanked by a short amino-terminal ectodomain and a large carboxy-terminal endodomain. In this study, we showed co-purification of the M protein from coronavirus infectious bronchitis vir...

to evaluate the effect of supplemental vitamin c (vc) levels on performance and immune response of broiler chicks, 300 day-old ross male broiler chicks were allocated to a completly randomized design of 5 treatments(500 and 1000 ppm in diet, 500 and 1000 ppm in drinking water and control). immunity was assessed as antibody production against infectious bronchitis virus (ibv) and newcastle disea...

The avian coronavirus Infectious bronchitis virus (IBV), like other coronaviruses, expresses several small nonstructural (ns) proteins in addition to those from gene 1 (replicase) and the structural proteins. These coronavirus ns genes differ both in number and in amino acid similarity between the coronavirus groups but show some concordance within a group or subgroup. The functions and require...

Avian infectious bronchitis virus (IBV), which causes respiratory disease in infected birds, belongs to coronavirus group 3. IBV encodes 15 nonstructural proteins (nsp2-nsp16) which play crucial roles in RNA transcription and genome replication. Nonstructural protein 9 (nsp9) has been identified as a protein that is essential to viral replication because of its single-stranded RNA-binding abili...

Concanavalin A-Sandwich ELISA (Con A-S-ELISA) was developed for the detection of infectious bronchitis virus (IBV) or chicken specific anti-viral antibodies. The antigen detection limit for the Con A-S-ELISA was 10(5,1) EID(50)/mL. Three homologous and four heterologous IBV strains were similarly detected. This assay was highly effective in detecting the virus after infected tissue homogenates ...

Viral pathogens cause devastating economic losses in poultry industries worldwide. The Caribbean region, which boasts some of the highest rates of poultry consumption in the world, is no exception. This review summarizes evidence for the circulation and spread of eight high-priority, economically important poultry viruses across the Caribbean region. Avian influenza virus (AIV), infectious bron...

Infectious bronchitis (IB) is a highly contagious disease involving mostly upper respiratory tract in chickens, leading to significant economic losses in the poultry industry worldwide. One of the major concerns regarding to IB is the emergence of new types of infectious bronchitis viruses (IBVs). The purpose of this study was to identify the IBVs isolated from Iranian broiler chickens with res...

A liquid phase blocking ELISA (LPB-ELISA) was developed for the detection and measurement of antibodies against infectious bronchitis virus (IBV). The purified and nonpurified virus used as antigen, the capture and detector antibodies, and the chicken hyperimmune sera were prepared and standardized for this purpose. A total of 156 sera from vaccinated and 100 from specific pathogen-free chicken...

Background and Aims: Avian infectious bronchitis virus (IBV) has a worldwide distribution and mutations occurring in the large viral genome of IBV have led to extensive antigenic variations among IBVs. This is the first study conducted to determine the complete membrane (M) gene sequences of different Iranian IBV genotypes. Materials and Methods: The M gene of three 793/B (IBKG1,6,7), one Mass...

Montassier M.F.S., Brentano L., Montassier H.J. & Richtzenhain L.J. 2008. Genetic grouping of avian infectious bronchitis virus isolated in Brazil, based on RT-PCR/RFLP analysis of the S1 gene. Pesquisa Veterinária Brasileira 28(3):190-194. Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando de Paiva 87, São Paulo, SP 05508-000, Brazil. E-mail: leonar...