The 70-kDa protoxin of Cry11A, a dipteran-specific insecticidal protein, was processed by trypsin into 36- and 32-kDa fragments. To investigate the potent function of the two processed fragments, a GST (Glutathione-S-transferase) fusion protein of each polypeptide was constructed. While neither the 36- nor the 32-kDa fragment was toxic to Culex pipiens larvae, coexpression of the two fragments ...

S. Gomathi and K.M. Subbu Rathinam* ABSTRACT To identify the insecticidal components of Saraca asoca bark were evaluated by standard protocol using the equipment Perkin-Elmer Gas Chromatography–Mass Spectrometry. The mass spectrum of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. The GC-MS analysis revealed the presence of ...

A series of insecticidal dihydroagarofuran sesquiterpene polyesters were isolated from the root bark of Chinese bittersweet (Celastrus angulatus Max). A previous study indicated that these compounds affect the digestive system of insects, and aminopeptidase N3 and V-ATPase have been identified as the most putative target proteins by affinity chromatography. In this study, the correlation betwee...

In this work, we report the genome sequencing of two Bacillus thuringiensis strains using Illumina next-generation sequencing technology (NGS). Strain Hu4-2, toxic to many lepidopteran pest species and to some mosquitoes, encoded genes for two insecticidal crystal (Cry) proteins, cry1Ia and cry9Ea, and a vegetative insecticidal protein (Vip) gene, vip3Ca2. Strain Leapi01 contained genes coding ...

The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is an important pest of citrus because it transmits plant pathogens responsible for a serious disease of citrus known as huanglongbing. Conventional insecticides are frequently used to manage ACP. Insecticidal soaps (hereafter ‘soaps’) are an insect control option labelled for commercial use as well as for use by ...

The insecticidal activity of aqueous extract of Panax notoginseng (Burkill) F. H. Chen (Araliaceae) flower buds against Aedes albopictus Skuse (Diptera Culicidae) larvae and pupae was tested and quali-quantitative profile of principal saponins of the active tested aqueous extract were investigated by LC-ESI/MS/MS. P. notoginseng aqueous extract (35% of saponins/dried plant extract) showed the h...

In the current study, the stem wood of Millettia ovalifolia was evaluated for antifungal, insecticidal and phytotoxic activities. The results revealed that all the fractions showed no antibacterial activity against the tested bacterial strains. In case of antifungal activity, only ethyl acetate (EA) and chloroform (C) fractions exhibited minimum inhibitory concentration both at 500 μg/ml agains...

In this study, in order to find novel biologically active pyrazole oxime compounds, a number of dichloro-allyloxy-phenol-containing pyrazole oximes were designed and synthesized according to the method of active group combination. All of the target compounds were confirmed by ¹H-NMR, (13)C-NMR and elemental analysis. In addition, bioassays showed that all of the newly synthesized compounds had ...

A simple and selective assay system was developed in the search for new insecticidal substances from Actinomycetales strains propagated on solid culture media. The strains were first tested for their ability to produce antimicrobial compounds. Only strains displaying weak or no activity were retained and screened in the insecticidal bioassay. Microbial solid cultures were given as food to larva...

Objective: To explore the phytochemical and antimicrobial activities as well as the insecticidal properties of the different sections of Ricinus communis (castor) plant in Mauritius. Methods: Qualitative and quantitative methods were used for the determination of phytochemicals in the crude leaves, pericarp, seeds, bark and root extracts obtained by using polar and non-polar solvents. The disc ...