We have compared the DNA sequences of several different examples of the duplicated polymerase I promoters that are found in rDNA spacers of Xenopus laevis. Although different spacers exhibit different amounts of transcription in vivo, this does not seem to be due to DNA sequence differences between spacer promoters. We have found that several different spacer promoters when subcloned and inject...

Stem rot was found for the first time on the Asiatic dayflower plant (Commelina communis L.) in Korea. A detailed description of this Korean specimen is given, along with its rDNA internal transcribed spacer sequence. The fungus was identified as Sclerotium rolfsii Saccardo based on mycological characteristics and molecular data.

Soft rot in apple caused by Rhizopus oryzae was found for the first time in Korea. A detailed description of the specimen is given along with its internal transcribed spacer rDNA sequence. The fungus was identified as Rhizopus oryzae based on the mycological characteristics, molecular data, and pathogenicity testing.

The order Saprolegniales includes pathogenic species of aquatic and terrestrial plants and aquatic animals including fish. These straminipilous fungi play an important role as a decomposing component which participates in the destruction processes of organic substrates and self-cleaning biological processes in freshwater ecosystems. In this study, samples of brown decayed twigs, leaves, woods a...

Fifty isolates of dry root rot pathogen Rhizoctonia bataticola collected from different agro-climatic zones in India were evaluated for the degree of subdivision in isolates level. The isolates showed variability in pathogenicity test. Genetic characteristics were analyzed based on the sequence of the rDNA-internal transcribed spacer (ITS) region. The phylogenetic tree based on rDNA-ITS analysi...

The recently described polyploid Saxifraga svalbardensis is endemic to the arctic archipelago of Svalbard. We investigated relationships among four closely related species of Saxifraga in Svalbard and tested three previously proposed hypotheses for the origin of S. svalbardensis: (1) differentiation from the morphologically and chromosomally variable polyploid S. cernua; (2) hybridization betwe...

Abstract Our studies on lignicolous aquatic fungi in Thailand, Sweden, and the UK resulted collection of three new Halobyssothecium species ( H . bambusicola , phragmitis versicolor ) assigned to Lentitheciaceae (Pleosporales, Dothideomycetes). Multi-loci phylogenetic analyses combined large subunit, small internal transcribed spacers ribosomal DNA, translation elongation factor 1-alpha sequenc...

The alignment of the 28S gene of several species of Pezizales allowed to select two pairs of primers able to amplify the internal transcribed spacer region of ribosomal DNA in mycorrhizal fungi, such as truffles. The higher yield of the amplification product demonstrates a better annealing of the new primers to the rDNA, as compared to the universal primers internal transcribed spacer 1 and int...

BACKGROUND AND PURPOSE By using advanced detection/identification methods, the list of emerging uncommon opportunistic yeast infections is rapidly expanding worldwide. Our aim in the present study was sequence-based species delineation of previously unidentified yeasts obtained from a clinically yeast collection. MATERIALS AND METHODS A total of twenty three out of the 855 (5.7%) yeast isolat...