The kinetic mechanism of the unidirectional H2-oxidizing hydrogenase from soybean nodule bacteroids has been investigated with highly purified enzyme. Measurements of the Km for H2 vary from 0.97 to 2.6 microM, and the Km for methylene blue varies from 6 to 17 microM. With H2 and methylene blue as substrates, the initial velocity patterns are intersecting. High levels of methylene blue are inhi...

Silicone polymers containing the light-activated antimicrobial agent methylene blue with or without gold nanoparticles were evaluated for their ability to reduce the microbial load on surfaces in a clinical environment. When irradiated with white light, polymers containing nanogold were more effective in this respect than those containing only methylene blue.

Radiographic contrast studies have been recommended to identify the thoracic duct (TD) and its branches before and after surgery for total occlusion. The macroscopic identification of the TD and its branches during surgery usually involves injection of methylene blue (MB). Radiographic contrast and methylene blue can be injected into different anatomical structures (lymph node, lymph vessel, s....

1. Cysteamine is oxidized to hypotaurine by an enzyme extracted from horse kidney, with sulphur or sulphide acting as a cofactor. It has been now found that, when the enzyme is omitted, sulphur and sulphide are able to catalyse the oxidation of cysteamine to cystamine by molecular oxygen. 2. Methylene blue may be used in catalytic amounts as a cofactor in the enzymic oxidation of cysteamine to ...

The existing rapid method for the determination of anionic surfactants in aqueous solutions relies on the association of the cationic methylene blue dye with the anionic surfactant to form an electroneutral complex that is subsequently extracted into chloroform and determined spectrophotometrically. This methylene blue active substance (MBAS) assay suffers from salt interference in estuarine an...

The aim of this study was to evaluate the effects of the laser radiation (685 nm) associated with photosensitizers on viability of different species of Candida genus. Suspensions of Candida albicans, Candida dubliniensis, Candida krusei and Candida tropicalis, containing 10(6) viable cells per milliliter were obtained with the aid of a Neubauer's chamber. From each species, 10 samples of the ce...

PURPOSE Sentinel lymph node biopsy (SLNB) in breast cancer patients with clinically negative axilla will ensure axillary dissection only for cases with lymph node metastasis and provide information about pathologic staging as accurate as the axillary dissection. It was shown that SLNB could be successfully performed regardless of the type of biopsy. The aim of this study was to investigate the ...

BACKGROUND/AIMS Postoperative adhesions following abdominopelvic surgery may cause significant morbidity. In this study, the effect of different doses of methylene blue in the formation of adhesions and the role of allopurinol in revealing the possible mechanism of action was evaluated. METHODS Seventy five male Wistar-Albino rats weighing 200g-220g. were divided into five groups of 15 rats e...

Background and Purpose: Methylene blue and 6-anilino,5,8-quinolinedione (LY83583) are used extensively to block activation of guanylate cyclase. Both agents generate oxygen radicals. Therefore, it appeared profitable to investigate whether the generation of oxygen radicals by these agents is responsible for the blockade of responses to nitrodilators that act via activation of guanylate cyclase ...

the nanocomposites of poly (acryl amide-co-acrylic acid) with different contents of naazeolite were prepared using ammonium persulfate as an initiator and n, n′-methylenebisacrylamide as the crosslinker. the morphology was characterized by sem and the structure ofnanocomposite materials was studied with xrd and ft-ir that showed the interaction betweenporous materials and poly (aam-co-aac). the...