glsA gene product, which is required for asymmetric division, and the regA gene product, which is required for terminal differentiation of somatic cells. More recently, they have also been used to show that the invA gene encodes a novel kinesin that drives inversion. What can Volvox tell us about the evolutionary origins of multicellularity and cellular differentiation? The family Volvocaceae, ...

Article history: Received on: 17/08/2016 Revised on: 08/09/2016 Accepted on: 07/10/2016 Available online: 29/11/2016 The present study has explored the culture-independent quantification of Salmonellae in selected food items by targeting invA gene using molecular beacon (MB) based real-time PCR assay. The assay could detect ten Genome Equivalents/PCR (GE/PCR) of reference strain S. typhimurium ...

OBJECTIVES This study aimed to investigate Salmonella Enteritidis strains isolated from human and food sources in the north of Morocco by means of phenotypic and genotypic methods. METHODOLOGY Fifteen isolates from humans and food were submitted to phage typing, XbaI-macrorestriction (pulsed field gel electrophoresis [PFGE]), enterobacterial repetitive intergenic consensus (ERIC-PCR), antimic...

BACKGROUND AND OBJECTIVE Wound infections are often difficult to treat due to various bacterial pathogens. Pseudomonas aeruginosa is one of the common invaders of open wounds. Precise diagnosis of this etiological agent in wound infections is of critical importance particularly in treatment of problematic cases. The existing diagnostic methods have certain limitations particularly related to sp...

Quantitative PCR (Q-PCR) is a fast and efficient tool to quantify target genes. In eukaryotic cells, quantitative reverse transcription-PCR (Q-RT-PCR) is also used to quantify gene expression, with stably expressed housekeeping genes as standards. In bacteria, such stable expression of housekeeping genes does not occur, and the use of DNA standards leads to a broad underestimation. Therefore, a...

We recently found that Yersinia pseudotuberculosis can be used as a model of persistent bacterial infections. We performed in vivo RNA-seq of bacteria in small cecal tissue biopsies at early and persistent stages of infection to determine strategies associated with persistence. Comprehensive analysis of mixed RNA populations from infected tissues revealed that Y. pseudotuberculosis undergoes tr...

Salmonella typhimurium definitive type 104 (DT104) is a virulent pathogen for humans and animals with many strains having multiple drug resistance characteristics. The organism typically carries resistance to ampicillin, chloramphenicol, florfenicol, streptomycin, sulfonamides, and tetracycline (ACSSuT-resistant). A multiplex PCR method was developed to simultaneously amplify four genes, florfe...

We have found that early in infection of the intracellular pathogen Listeria monocytogenes in Madin-Darby canine kidney epithelial cells expressing actin conjugated to green fluorescent protein, F-actin rapidly assembles ( 25 s) and disassembles ( 30 s) around the bacteria, a phenomenon we call flashing. L. monocytogenes strains unable to perform actin-based motility or unable to escape the pha...