Chemical modification of α-amylase from locale bacteria isolate Bacillus subtilis ITBCCB148 using citraconic anhydride has been performed. increase thermal stability of the enzyme has successfully been done. The results showed that the modified enzymes with citraconic anhydride with modification degree of 45, 73, 82, 83, and 88% have similar optimum pH of 5.5 and optimum temperature of 60°C; ki...

Cysteine sulfenic acids in proteins can be identified by their ability to form adducts with dimedone, but this reagent imparts no spectral or affinity tag for subsequent analyses of such tagged proteins. Given its similar reactivity toward cysteine sulfenic acids, 1, 3-cyclohexadione was synthetically modified to an alcohol derivative and linked to fluorophores based on isatoic acid and 7-metho...

UNLABELLED BACKGROUND Microwave irradiation as an alternative heat source is now a well-known method in synthetic chemistry. Microwave heating has emerged as a powerful technique to promote a variety of chemical reactions, offering reduced pollution, low cost and offer high yields together with simplicity in processing and handling. On the other hand, copolymers containing both hydrophilic a...

for allowing separation of its constituents. These reagents are likely to be specific for amino groups, and the aminoacyl bonds they form (Dixon & Perham, 1968) appeared sufficiently labile to allow their hydrolysis under conditions not deleterious to Na,K-ATPase activity. Microsomal suspensionswere prepared from frozen ox cerebral cortex essentially by the procedure of Schwartz, Bachelard & Mc...

The purpose of this research was to find a derivative of 3-hydroxy-3-methyIglutaric acid which would prevent the reduction of 3-hydroxy-3-methylglutaric Coenzyme A to mevalonic acid by competitively inhibiting the enzyme 3-hydroxy-3-methylglutaric Coenzyme A reductase* Reduction of this reaction would inhibit the synthesis of cholesterol at a strategic site since this is the first irreversible ...

Bio-based furanics can be aromatized efficiently by sequential Diels-Alder (DA) addition and hydrogenation steps followed by tandem catalytic aromatization. With a combination of zeolite H-Y and Pd/C, the hydrogenated DA adduct of 2-methylfuran and maleic anhydride can thus be aromatized in the liquid phase and, to a certain extent, decarboxylated to give high yields of the aromatic products 3-...

The aim was to study the ability of bioadhesive cyclodextrin-poly(anhydride) nanoparticles as carriers for the oral delivery of atovaquone (ATO). In order to increase the loading capacity of ATO by poly(anhydride) nanoparticles, the following oligosaccharides were assayed: 2-hydroxypropyl-β-cyclodextrin (HPCD), 2,6-di-O-methyl-β-cyclodextrin (DCMD), randomly methylated-β-cyclodextrin (RMCD) and...

Epon epoxy resin was introduced as an embedding medium for biological electron microscopy largely through the independent efforts of Finck (1) and Luft (2). The usual recipe involves three components: Epon 812 resin (Epikote 812, in England), an anhydride hardener, and an accelerator such as DMP-30. Since the combination of Epon with dodecenyl succinic anhydride (DDSA) was thought to produce a ...

The reaction of 4-methyl-2-phenyl-1,2-dihydro-6-oxo-5-pyridine- carbonitrile (1) with arylidene malononitrile afforded isoquinoline derivatives 2a,b. 6-Chloro-4-methyl-2-phenyl-5-pyridinecarbonitile (3) obtained by chlorination of compound 1 with phosphoryl chloride was converted into 6-amino-4-methyl-2-phenyl-5-pyridinecarbonitrile (4) and 6-hydrazido-4-methyl-2-phenyl-5-pyridinecarbonitrile (...